The Application of Fluorine-Containing Reagents in Structural Proteomics.,Angewandte Chemie International Edition

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The Application of Fluorine-Containing Reagents in Structural Proteomics.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2019-10-07 , DOI: 10.1002/anie.201907662
Ming Cheng ₁ , Chunyang Guo ₁ , Michael L Gross ₁

Affiliation

Structural proteomics refers to large-scale mapping of protein structures in order to understand the relationship between protein sequence, structure, and function. Chemical labeling, in combination with mass-spectrometry (MS) analysis, have emerged as powerful tools to enable a broad range of biological applications in structural proteomics. The key to success is a biocompatible reagent that modifies a protein without affecting its high-order structure. Fluorine, well-known to exert profound effects on the physical and chemical properties of reagents, should have an impact on structural proteomics. In this Minireview, we describe several fluorine-containing reagents that can be applied in structural proteomics. We organize their applications around four MS-based techniques: a) affinity labeling, b) activity-based protein profiling (ABPP), c) protein footprinting, and d) protein cross-linking. Our aim is to provide an overview of the research, development, and application of fluorine-containing reagents in protein structural studies.

中文翻译：

含氟试剂在结构蛋白质组学中的应用。

结构蛋白质组学是指对蛋白质结构进行大规模绘图，以了解蛋白质序列、结构和功能之间的关系。化学标记与质谱 (MS) 分析相结合，已成为实现结构蛋白质组学中广泛生物应用的强大工具。成功的关键是一种生物相容性试剂，可以修饰蛋白质而不影响其高级结构。众所周知，氟会对试剂的物理和化学性质产生深远的影响，它也会对结构蛋白质组学产生影响。在这篇小综述中，我们描述了几种可应用于结构蛋白质组学的含氟试剂。我们围绕四种基于 MS 的技术组织他们的应用：a) 亲和标记、b) 基于活性的蛋白质分析 (ABPP)、c) 蛋白质足迹和 d) 蛋白质交联。我们的目的是概述含氟试剂在蛋白质结构研究中的研究、开发和应用。

更新日期：2020-01-16

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