An acute inflammatory response, cellular infiltrates, anemia, hemorrhage and endogenous fibrinolysis activation were previously described in C57BL/6 mice injected with M. tener tener venom (Mtt). As the endothelium and innate immunity may participate in these disturbances and due to our poor understanding of the alterations produced by these venoms when the neurotoxic component is not predominant, we evaluated the effects in an in vitro model. At 24 h, the release of pro-inflammatory mediators was detected in peritoneal macrophages. At different times, the release of pro-inflammatory (TNF-α, IL-6, NO and E-Selectin), pro-coagulant (vWF and TF) and pro-fibrinolytic (uPA) mediators were seen in liver sinusoidal endothelial cells (LSECs). These results suggest that Mtt venom activates macrophages and endothelium, thus inducing the release of mediators, such as TNF-α, that orchestrate the acute inflammatory response and the later infiltration of mononuclear cells into liver in C57BL/6 mice. In addition, endothelium activation promotes TF expression, which may in turn modulate the inflammatory and hemostatic response. These findings suggest crosstalk between inflammation and hemostasis in the alterations observed in Micrurus envenomation, where the neurotoxic manifestations do not predominate.

中文翻译：

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内皮细胞和巨噬细胞活化在C57BL / 6小鼠中由细支ic虫的毒液诱导的改变中的作用。

先前在注射特里氏疟原虫毒液（Mtt）的C57BL / 6小鼠中描述了急性炎症反应，细胞浸润，贫血，出血和内源性纤溶激活。由于内皮和先天免疫可能参与这些紊乱，并且由于当我们对神经毒成分不占优势时，我们对这些毒液产生的变化了解不多，因此我们在体外模型中评估了这种作用。在24小时时，在腹膜巨噬细胞中检测到促炎性介质的释放。在不同的时间，在肝窦内皮细胞中观察到促炎性因子（TNF-α，IL-6，NO和E-选择素），促凝剂（vWF和TF）和促纤溶剂（uPA）的释放。 LSEC）。这些结果表明，Mtt毒液会激活巨噬细胞和内皮细胞，因此诱导了C57BL / 6小鼠中介导急性炎症反应的介质（如TNF-α）的释放以及后来单核细胞向肝脏的浸润。另外，内皮细胞的活化促进了TF的表达，而TF的表达反过来又可以调节炎症反应和止血反应。这些发现表明，在弥漫性毒化中观察到的变化中，炎症和止血之间存在串扰，在该处神经毒性表现并不占主导地位。