Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.,The Plant Cell

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Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-10-07 , DOI: 10.1105/tpc.19.00263
Yi Sun ₁ , Melissa Valente-Paterno ₁ , Shiva Bakhtiari ₁ , Christopher Law ₂ , Yu Zhan ₁ , William Zerges ₃

Affiliation

Intracellular processes can be localized for efficiency or regulation. For example, localized mRNA translation by chloroplastic ribosomes occurs in the biogenesis of PSII, one of the two photosystems of the photosynthetic electron transport chain in the chloroplasts of plants and algae. The biogenesis of PSI and PSII requires the synthesis and assembly of their constituent polypeptide subunits, pigments, and cofactors. Although these biosynthetic pathways are well characterized, less is known about when and where they occur in developing chloroplasts. Here, we used fluorescence microscopy in the unicellular alga Chlamydomonas reinhardtii to reveal spatiotemporal organization in photosystem biogenesis. We focused on translation by chloroplastic ribosomes and chlorophyll biosynthesis in two developmental contexts of active photosystem biogenesis: (1) growth of the mature chloroplast and (2) greening of a nonphotosynthetic chloroplast. The results reveal that a translation zone is the primary location of the biogenesis of PSI and PSII. This discretely localized region within the chloroplast contrasts with the distributions of photosystems throughout this organelle and, therefore, is likely a hub where anabolic pathways converge for photosystem biogenesis.plantcell;31/12/3057/FX1F1fx1.

中文翻译：

光系统生物发生位于衣藻叶绿体的翻译区。

细胞内过程可以局部化以提高效率或调节。例如，叶绿体核糖体的局部 mRNA 翻译发生在 PSII 的生物发生中，PSII 是植物和藻类叶绿体中光合电子传递链的两个光系统之一。 PSI 和 PSII 的生物合成需要其组成多肽亚基、色素和辅因子的合成和组装。尽管这些生物合成途径已得到很好的表征，但人们对它们在叶绿体发育过程中发生的时间和地点知之甚少。在这里，我们使用单细胞藻类莱茵衣藻的荧光显微镜来揭示光系统生物发生中的时空组织。我们专注于叶绿体核糖体和叶绿素生物合成在主动光系统生物发生的两个发育背景中的翻译：（1）成熟叶绿体的生长和（2）非光合作用叶绿体的绿化。结果表明翻译区是PSI和PSII生物发生的主要位置。叶绿体内的这个离散局部区域与整个细胞器中光系统的分布形成对比，因此，可能是光系统生物发生的合成代谢途径汇聚的中心。plantcell;31/12/3057/FX1F1fx1。

更新日期：2019-12-11

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