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Rectification of protein translocation in truncated pyramidal nanopores.
Nature Nanotechnology ( IF 38.1 ) Pub Date : 2019-10-07 , DOI: 10.1038/s41565-019-0549-0 Shuangshuang Zeng 1 , Chenyu Wen 1 , Paul Solomon 2 , Shi-Li Zhang 1 , Zhen Zhang 1
Nature Nanotechnology ( IF 38.1 ) Pub Date : 2019-10-07 , DOI: 10.1038/s41565-019-0549-0 Shuangshuang Zeng 1 , Chenyu Wen 1 , Paul Solomon 2 , Shi-Li Zhang 1 , Zhen Zhang 1
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Solid-state nanopore technology presents an emerging single-molecule-based analytical tool for the separation and analysis of nanoparticles. Different approaches have been pursued to attain the anticipated detection performance. Here, we report the rectification behaviour of protein translocation through silicon-based truncated pyramidal nanopores. When the size of translocating proteins is comparable to the smallest physical constriction of the nanopore, the frequency of translocation events observed is lower for proteins that travel from the larger to the small opening of the nanopore than for those that travel in the reverse direction. When the proteins are appreciably smaller than the nanopore, an opposite rectification in the frequency of translocation events is evident. The maximum rectification factor achieved is around ten. Numerical simulations reveal the formation of an electro-osmotic vortex in such asymmetric nanopores. The vortex-protein interaction is found to play a decisive role in rectifying the translocation in terms of polarity and amplitude. The reported phenomenon can be potentially exploitable for the discrimination of various nanoparticles.
中文翻译：
截断的锥体纳米孔中蛋白质转运的校正。
固态纳米孔技术提出了一种新兴的基于单分子的分析工具，用于分离和分析纳米颗粒。已经采取了不同的方法来获得预期的检测性能。在这里，我们报告通过基于硅的截断的金字塔形纳米孔蛋白易位的整流行为。当易位蛋白的大小可与纳米孔的最小物理收缩相媲美时，从纳米孔的较大开口到较小开口行进的蛋白质的观察到的易位事件的频率要比反向方向行进的蛋白质的观察到的易位事件的频率低。当蛋白质明显小于纳米孔时，易位事件的频率将发生相反的整流。达到的最大整流系数约为十。数值模拟表明在这种不对称的纳米孔中形成了电渗涡流。发现涡旋-蛋白质相互作用在纠正极性和振幅方面的易位中起决定性作用。所报道的现象可潜在地用于区分各种纳米颗粒。
更新日期：2019-10-07
中文翻译：
截断的锥体纳米孔中蛋白质转运的校正。
固态纳米孔技术提出了一种新兴的基于单分子的分析工具，用于分离和分析纳米颗粒。已经采取了不同的方法来获得预期的检测性能。在这里，我们报告通过基于硅的截断的金字塔形纳米孔蛋白易位的整流行为。当易位蛋白的大小可与纳米孔的最小物理收缩相媲美时，从纳米孔的较大开口到较小开口行进的蛋白质的观察到的易位事件的频率要比反向方向行进的蛋白质的观察到的易位事件的频率低。当蛋白质明显小于纳米孔时，易位事件的频率将发生相反的整流。达到的最大整流系数约为十。数值模拟表明在这种不对称的纳米孔中形成了电渗涡流。发现涡旋-蛋白质相互作用在纠正极性和振幅方面的易位中起决定性作用。所报道的现象可潜在地用于区分各种纳米颗粒。
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