Quantification of micropolarity and microviscosity of aggregation and salt-induced gelation of sodium deoxycholate (NaDC) using Nile red fluorescence†,Photochemical & Photobiological Sciences

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Quantification of micropolarity and microviscosity of aggregation and salt-induced gelation of sodium deoxycholate (NaDC) using Nile red fluorescence†
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2019-10-02 , DOI: 10.1039/c9pp00293f
Jitendriya Swain _{1,

2,

3,

4} , Jhili Mishra _{1,

2,

3,

4} , Goutam Ghosh _{1,

2,

3,

4} , Ashok Kumar Mishra _{1,

2,

3,

4}

Affiliation

This study reports the utility of the hydrophobic probe Nile Red (NR) to understand the concentration induced microenvironmental changes of sodium deoxycholate (NaDC) bile salt from the premicellar to postmicellar range. The spectroscopic properties like absorbance value, fluorescence intensity and fluorescence lifetime of NR are significantly sensitive towards different states of aggregation of NaDC bile salt. The critical aggregation concentrations of different states (dimer to primary micellar aggregates (1.0 mM), secondary micellar aggregates (7.0 mM), and higher micellar aggregates (14 mM)) have been determined from the absorbance value and fluorescence intensity measurements. The E_T(30) polarity parameter values suggest a considerable decrease in the micropolarity with an increase in NaDC concentrations. Furthermore, the spectroscopic properties of NR are also sensitive towards the NaCl induced gelation process of NaDC bile salt. Changes in the micropolarity and microviscosity of the NaDC + NaCl mixed system have been estimated using the emission maximum value (cm⁻¹) and fluorescence lifetime values of NR with an increase in the NaCl concentration. Microviscosity of the medium increases from ∼19 mPa s to ∼26 mPa s from the sol phase to the gel phase. Temperature dependence of both size and phase changes of the NaDC + NaCl (30 mM + 1 M) gel network has been studied using differential scanning calorimetry and dynamic light scattering studies. Temperature induced polarity and microviscosity changes of the NaDC + NaCl (30 mM + 1 M) gel network have also been studied using the fluorescence properties of NR.

中文翻译：

尼罗红荧光法定量测定脱氧胆酸钠（NaDC）的聚集和盐诱导的胶凝作用的微极性和微粘度†

这项研究报告了疏水探针尼罗红（NR）的效用，以了解浓度引起的脱氧胆酸钠（NaDC）胆汁盐的微环境变化，从前胶束到后胶束。NR的光谱特性（如吸光度值，荧光强度和荧光寿命）对NaDC胆盐的不同聚集状态非常敏感。从吸光度值和荧光强度测量值可以确定不同状态的临界聚集浓度（二聚体至一级胶束聚集体（1.0 mM），二级胶束聚集体（7.0 mM）和较高的胶束聚集体（14 mM））。该Ë _Ť（30）极性参数值表明，随着NaDC浓度的增加，微极性显着降低。此外，NR的光谱性质对NaCl诱导的NaDC胆盐的胶凝过程也很敏感。已使用发射最大值（cm ^-1）和NR的荧光寿命值随NaCl浓度的增加而增加。从溶胶相到凝胶相，介质的微粘度从〜19 mPa s增加到〜26 mPa s。NaDC + NaCl（30 mM +1 M）凝胶网络的大小和相变的温度依赖性已通过差示扫描量热法和动态光散射研究进行了研究。利用NR的荧光特性，还研究了温度诱导的NaDC + NaCl（30 mM +1 M）凝胶网络的极性和微粘度变化。

更新日期：2019-11-06

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