De novo transcriptome characterisation of two auxin-related genes associated with plant growth habit in Astragalus adsurgens Pall.,Plant Biology

当前位置： X-MOL 学术 › Plant Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

De novo transcriptome characterisation of two auxin-related genes associated with plant growth habit in Astragalus adsurgens Pall.
Plant Biology ( IF 4.2 ) Pub Date : 2019-11-03 , DOI: 10.1111/plb.13052
L Ma ₁ , D Yi ₁ , W Gong _{1,

2} , P Gong _{1,

3} , Z Wang ₁

Affiliation

Astragalus adsurgens Pall., a perennial legume native to China, is commonly used as a forage crop. And it has great value for sustainable development of grasslands in arid and semi arid regions. However, to date, little is known regarding the A. adsurgens genome, and no studies have determined whether it would be possible to improve the germplasm of A. adsurgens through genetic modification. In this study, we used an RNA-seq protocol to generate a de novo transcriptome including 151,516 unigenes of A. adsurgens. We compared the transcriptomes of A. adsurgens having different growth habits (prostrate/erect) and identified 14,133 single nucleotide polymorphism sites (SNP) in 8,139 unigenes. Differential expression gene (DEG) analysis suggested that 10,982 unigenes were up-regulated in the prostrate plant relative to the erect plant, while 10,607 unigenes were down-regulated. Of the 21,589 DEG, Unigene72782_All (LAX4) and CL12494.Contig3_All (TIR1), an auxin transporter gene and an auxin transport inhibitor gene, respectively, were predicted to influence the growth habit of A. adsurgens, which were verified by qRT-PCR in these phenotypes. These results suggest that auxin transport was more active in the prostrate plant than in the erect plant, resulting in asymmetric distribution of auxin that affects the growth habit of A. adsurgens. Overall, this study may provide a basis for future research on key genes in A. adsurgens and may deepen our understanding of the molecular mechanisms regulating plant growth habit.

中文翻译：

从头转录组表征两个与植物生长习性相关的生长素相关基因在黄芪刺耳草颇尔。

多年生的豆科植物黄芪（Astragalus adsurgens Pall。）通常用作草料作物。对于干旱和半干旱地区的草地可持续发展具有重要价值。然而，迄今为止，关于A.adsurgens的基因组知之甚少，并且还没有研究确定是否有可能通过基因修饰来改善A. adsurgens的种质。在这项研究中，我们使用了RNA-seq协议来生成从头转录组，其中包括151,516个A. adsurgens单基因。我们比较了具有不同生长习惯（pro /直立）的A. adsurgens的转录组，并在8,139个单基因中鉴定出14,133个单核苷酸多态性位点（SNP）。差异表达基因（DEG）分析表明，strate实植物相对于直立植物有10,982个单基因被上调，而10个单基因被上调。607个单基因被下调。在21,589个DEG中，Unigene72782_All（LAX4）和CL12494.Contig3_All（TIR1）中的生长素转运蛋白基因和生长素转运抑制剂基因分别被预测会影响A. adsurgens的生长习性，这在qRT-PCR中得到了证实。这些表型。这些结果表明，在生长前的植物中，生长素的运输比在直立的植物中更活跃，导致生长素的不对称分布，影响了A. adsurgens的生长习性。总体而言，这项研究可能为今后对A. adsurgens中关键基因的研究提供基础，并可能加深我们对调节植物生长习性的分子机制的理解。分别被预测会影响A. adgesns的生长习性，这在这些表型中通过qRT-PCR进行了验证。这些结果表明，在生长前的植物中，生长素的运输比在直立的植物中更活跃，导致生长素的不对称分布，影响了A. adsurgens的生长习性。总体而言，这项研究可能为今后对A. adsurgens中关键基因的研究提供基础，并可能加深我们对调节植物生长习性的分子机制的理解。分别被预测会影响A. adgesns的生长习性，这在这些表型中通过qRT-PCR进行了验证。这些结果表明，在生长前的植物中，生长素的运输比在直立的植物中更为活跃，导致生长素的不对称分布，影响了A. adsurgens的生长习性。总体而言，这项研究可能为今后对A. adsurgens中关键基因的研究提供基础，并可能加深我们对调节植物生长习性的分子机制的理解。

更新日期：2019-11-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11