OBJECTIVE The aim of this study was to evaluate the penetration depth of silver into sound and demineralized dentin after application of silver diamine fluoride (SDF). METHODS Two hundred and eighty-eight dentin specimens were used. The specimens were divided into 3 groups: (1) sound dentin (control), (2) 30min EDTA-treated dentin; and (3) 13h EDTA-treated dentin. SDF was applied to all specimens. Each group was divided into 3 subgroups according to storage time into: 24h, 2 weeks and 1-year storage time. Each subgroup was further divided into four subgroups (n=8) according to different examinations as optical microscope (OM) observation, scanning electron microscopic (SEM) observation, elemental analysis with energy dispersive spectroscopy (EDS) and Micro-PIXE test. RESULTS The OM showed discoloration in the superficial layer after 24h and keep extending deeper after 2 weeks and 1-year. SEM showed silver crystals within dentinal tubules after 2 weeks and 1-year. EDS analysis can detect silver penetration only in the 1-year group reaching around 1200μm inside dentin. Micro-PIXE test detected silver at all time intervals, confirming the EDS depth results. SIGNIFICANCE It can be concluded that silver ions can completely infiltrate the demineralized dentin lesion with further penetration into the underlying mineralized dentin.

中文翻译：

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应用SDF后银渗透入声音/脱矿质牙本质的形态和元素分析。

目的本研究的目的是评估在应用二胺氟化银（SDF）后，银在纯净和脱矿质牙本质中的渗透深度。方法采用288个牙本质标本。标本分为3组：（1）声质牙本质（对照组），（2）EDTA处理30min的牙本质。（3）EDTA处理的13h牙本质。将SDF应用于所有标本。根据保存时间，将每个组分为3个子组：24h，2周和1年保存时间。根据光学显微镜（OM）观察，扫描电子显微镜（SEM）观察，能量色散光谱（EDS）元素分析和Micro-PIXE测试等不同检查，将每个亚组进一步分为四个亚组（n = 8）。结果OM在24h后显示浅表层变色，并在2周和1年后继续延伸得更深。SEM在2周和1年后显示出牙本质小管内的银晶体。EDS分析只能检测到在1年组中银渗透到牙本质内部约1200μm的情况。Micro-PIXE测试在所有时间间隔都检测到银，从而确认了EDS深度结果。意义可以得出结论，银离子可以完全渗透到脱矿质的牙本质病灶中，并进一步渗透到下面的矿化牙质中。确认EDS深度结果。意义可以得出结论，银离子可以完全渗透到脱矿质的牙本质病灶中，并进一步渗透到下面的矿化牙质中。确认EDS深度结果。意义可以得出结论，银离子可以完全渗透到脱矿质的牙本质病灶中，并进一步渗透到下面的矿化牙质中。