A major goal in structural biology is to unravel how molecular machines function in detail. To that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological assemblies in a near natural environment. Based on methyl TROSY methods, it is now possible to record high-quality data on complexes that are far over 100 kDa in molecular weight. In this review, we discuss the theoretical background of methyl TROSY spectroscopy, the information that can be extracted from methyl TROSY spectra and approaches that can be used to assign methyl resonances in large complexes. In addition, we touch upon insights that have been obtained for a number of challenging biological systems, including the 20S proteasome, the RNA exosome, molecular chaperones and G-protein-coupled receptors. We anticipate that methyl TROSY methods will be increasingly important in modern structural biology approaches, where information regarding static structures is complemented with insights into conformational changes and dynamic intermolecular interactions.

中文翻译：

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甲基 TROSY 光谱：研究具有挑战性的生物系统的通用 NMR 方法

结构生物学的一个主要目标是阐明分子机器的详细功能。为此，溶液态核磁共振光谱非常适合，因为它能够在近自然环境中研究生物组装。基于甲基 TROSY 方法，现在可以记录分子量远超过 100 kDa 的复合物的高质量数据。在这篇综述中，我们讨论了甲基 TROSY 光谱的理论背景、可以从甲基 TROSY 光谱中提取的信息以及可用于分配大型复合物中甲基共振的方法。此外，我们触及了许多具有挑战性的生物系统的见解，包括 20S 蛋白酶体、RNA 外泌体、分子伴侣和 G 蛋白偶联受体。