Recent studies have shown that global translational reprogramming is an early activation event in pattern-triggered immunity, when plants recognize microbe-associated molecular patterns. However, it is not fully known whether translational regulation also occurs in subsequent immune responses, such as effector-triggered immunity (ETI). In this study, we performed genome-wide ribosome profiling in Arabidopsis upon RPS2-mediated ETI activation and discovered that specific groups of genes were translationally regulated, mostly in coordination with transcription. These genes encode enzymes involved in aromatic amino acid, phenylpropanoid, camalexin, and sphingolipid metabolism. The functional significance of these components in ETI was confirmed by genetic and biochemical analyses. Our findings provide new insights into diverse translational regulation of plant immune responses and demonstrate that translational coordination of metabolic gene expression is an important strategy for ETI.

最近的研究表明，当植物识别微生物相关的分子模式时，全局翻译重编程是模式触发免疫的早期激活事件。然而，尚不清楚翻译调节是否也发生在随后的免疫反应中，例如效应触发免疫 (ETI)。在这项研究中，我们在拟南芥中进行了全基因组核糖体分析在 RPS2 介导的 ETI 激活后，发现特定基因组受到翻译调节，主要与转录协调。这些基因编码参与芳香族氨基酸、苯丙素、camalexin 和鞘脂代谢的酶。这些成分在 ETI 中的功能意义已通过遗传和生化分析得到证实。我们的研究结果为植物免疫反应的多种翻译调控提供了新的见解，并证明代谢基因表达的翻译协调是 ETI 的重要策略。