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A quinoline-based fluorescent probe for selective detection and real-time monitoring of copper ions - a differential colorimetric approach.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2019-11-07 , DOI: 10.1039/c9pp00247b Atika Farhi 1 , Farha Firdaus 2 , Haris Saeed 3 , Anzar Mujeeb 3 , Mohammad Shakir 1 , Mohammad Owais 3
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2019-11-07 , DOI: 10.1039/c9pp00247b Atika Farhi 1 , Farha Firdaus 2 , Haris Saeed 3 , Anzar Mujeeb 3 , Mohammad Shakir 1 , Mohammad Owais 3
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A quinoline moiety was used as a building block for designing a probe for the selective detection of copper ions in a partially aqueous medium. We have developed a molecular sensing system which gives insight into the complex physiological and redox aspects of labile copper. The probe provides a colorimetric approach for distinguishing cuprous and cupric ions along with their simultaneous discrimination from other metal ions in the visible range of the spectrum. The chemosensor showed a remarkable fluorescence enhancement along with a significant bathochromic shift of about 35 nm. The detection limit of the probe was found to be 1.03 μM which is optimally favorable to be applied in real-time monitoring. Fabrication of paper strips with the probe was done to detect the presence of cuprous ions in the real sample. The value of the binding constant (1.37 × 104 M-1) suggests stable complex formation between the metal ion and the sensing probe. The photoluminescence and structural aspects of the chemosensor were characterized by using fluorescence, absorption, ESI-MS, and 1H NMR spectroscopy. Furthermore, the cytotoxic nature and bioimaging properties of the probe were interpreted in vitro on RAW 264.7 macrophage cell lines and peripheral blood mononuclear cells (PBMCs) respectively.
中文翻译：
一种基于喹啉的荧光探针，用于铜离子的选择性检测和实时监控-差分比色法。
喹啉部分用作构建模块，以设计用于选择性检测部分水性介质中铜离子的探针。我们已经开发了一种分子传感系统，可以深入了解不稳定铜的复杂生理和氧化还原方面。该探针提供了一种比色法，用于区分亚铜和铜离子，以及它们与光谱可见范围内的其他金属离子的同时鉴别。化学传感器显示出显着的荧光增强以及约35 nm的显着红移。发现探针的检出限为1.03μM，最适合用于实时监测。用探针制造纸条以检测真实样品中亚铜离子的存在。结合常数（1.37×104 M-1）的值表明在金属离子和传感探针之间形成稳定的络合物。化学传感器的光致发光和结构方面通过使用荧光，吸收，ESI-MS和1H NMR光谱进行了表征。此外，分别在RAW 264.7巨噬细胞系和外周血单核细胞（PBMC）上解释了该探针的细胞毒性性质和生物成像性质。
更新日期：2019-12-04
中文翻译：
一种基于喹啉的荧光探针，用于铜离子的选择性检测和实时监控-差分比色法。
喹啉部分用作构建模块，以设计用于选择性检测部分水性介质中铜离子的探针。我们已经开发了一种分子传感系统，可以深入了解不稳定铜的复杂生理和氧化还原方面。该探针提供了一种比色法，用于区分亚铜和铜离子，以及它们与光谱可见范围内的其他金属离子的同时鉴别。化学传感器显示出显着的荧光增强以及约35 nm的显着红移。发现探针的检出限为1.03μM，最适合用于实时监测。用探针制造纸条以检测真实样品中亚铜离子的存在。结合常数（1.37×104 M-1）的值表明在金属离子和传感探针之间形成稳定的络合物。化学传感器的光致发光和结构方面通过使用荧光，吸收，ESI-MS和1H NMR光谱进行了表征。此外，分别在RAW 264.7巨噬细胞系和外周血单核细胞（PBMC）上解释了该探针的细胞毒性性质和生物成像性质。
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