Rhodosporidium toruloides is an oleaginous yeast that can accumulate up to 70% of its dry biomass as lipids and can use C5 and C6 sugars as a carbon source, that are present in hydrolysates from different lignocellulosic biomass. R. toruloides can be an alternative for the valorization of forestry residues and the obtention of higher value-added products. In this study, five strains of R. toruloides were evaluated to find the most efficient yeast strain for C5 and C6 sugars utilization, using lignocellulosic hydrolysates as a culture media. Sugar consumption was similar between all strains. Nevertheless, it was different among C6 and C5 hydrolysates, with maximum sugar utilization of 98%, and 60% for C6 and C5 hydrolysate, respectively. Among the studied strains, the highest lipid production was observed in R. toruloides-1588 with 23.33 g of lipids/L in C6 hydrolysate after 112 h. Whereas, highest lipid production in C5 hydrolysate was observed in R. toruloides-7191 with 14.67 g of lipids/L after 120 h. Predominantly, fatty acids for R. toruloides-1588 was oleic, palmitic, and stearic acids and pentadecanoic, palmitic, and heptadecanoic acids for R. toruloides-7191. Moreover, both strains grew and produced lipids in the presence of inhibitor compounds, such as levulinic acid (13.88 mgL⁻¹), 5-HMF (80.86 mgL⁻¹), furfural (153.54 mgL⁻¹), vanillin (17.17 mgL⁻¹), vanillic acid (85.25 mgL⁻¹), syringaldehyde (41.83 mgL⁻¹), ferulic acid (1.66 mgL⁻¹), and 4-hydroxybenzoic acid (0.85 mgL⁻¹). The study revealed that R. toruloides-1588 and R. toruloides-7191 are promising strains for C5 and C6 sugar utilization from lignocellulosic hydrolysates for lipid production.

球形假单胞菌是一种油脂性酵母，最多可以将其干生物质的70％积累为脂质，并可以使用C5和C6糖作为碳源，这些糖存在于来自不同木质纤维素生物质的水解物中。R. toruloides可以替代林业残留物和获得更高附加值的产品。在这项研究中，五个R. toruloides菌株使用木质纤维素水解产物作为培养基，对酵母菌进行评估以找到最有效的利用C5和C6糖的酵母菌株。所有菌株之间的糖消耗量相似。但是，C6和C5水解产物之间存在差异，最大糖利用率分别为98％和60％（对于C6和C5水解产物）。在所研究的菌株中，在112小时后，在R. toruloides -1588中观察到最高的脂质产量，其中C6水解产物中的脂质为23.33 g / L。而在120小时后，在R. toruloides -7191中观察到C5水解产物的最高脂质产量，其中脂质为14.67 g / L。R. toruloides -1588的脂肪酸主要为油酸，棕榈酸和硬脂酸，而十五碳酸，棕榈酸和十七碳酸为R. toruloides -7191。此外，两种菌株在抑制剂化合物如乙酰丙酸（13.88 mgL ^-1），5-HMF（80.86 mgL ^-1），糠醛（153.54 mgL ^-1），香兰素（17.17 mgL ^-1）的存在下生长并产生脂质。），香草酸（85.25 mgL ^-1），丁香醛（41.83 mgL ^-1），阿魏酸（1.66 mgL ^-1）和4-羟基苯甲酸（0.85 mgL ^-1）。该研究显示，R.酵母菌-1588和R.酵母菌-7191是有希望的，从生产油脂木质纤维素水解C5和C6糖利用的菌株。