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Lipid production in Rhodosporidium toruloides using C-6 and C-5 wood hydrolysate: A comparative study
Biomass & Bioenergy ( IF 5.8 ) Pub Date : 2019-09-27 , DOI: 10.1016/j.biombioe.2019.105355
Carlos S. Osorio-González , Krishnamoorthy Hegde , Pedro Ferreira , Satinder Kaur Brar , Azadeh Kermanshahipour , Carlos Ricardo Soccol , Antonio Avalos-Ramírez

Rhodosporidium toruloides is an oleaginous yeast that can accumulate up to 70% of its dry biomass as lipids and can use C5 and C6 sugars as a carbon source, that are present in hydrolysates from different lignocellulosic biomass. R. toruloides can be an alternative for the valorization of forestry residues and the obtention of higher value-added products. In this study, five strains of R. toruloides were evaluated to find the most efficient yeast strain for C5 and C6 sugars utilization, using lignocellulosic hydrolysates as a culture media. Sugar consumption was similar between all strains. Nevertheless, it was different among C6 and C5 hydrolysates, with maximum sugar utilization of 98%, and 60% for C6 and C5 hydrolysate, respectively. Among the studied strains, the highest lipid production was observed in R. toruloides-1588 with 23.33 g of lipids/L in C6 hydrolysate after 112 h. Whereas, highest lipid production in C5 hydrolysate was observed in R. toruloides-7191 with 14.67 g of lipids/L after 120 h. Predominantly, fatty acids for R. toruloides-1588 was oleic, palmitic, and stearic acids and pentadecanoic, palmitic, and heptadecanoic acids for R. toruloides-7191. Moreover, both strains grew and produced lipids in the presence of inhibitor compounds, such as levulinic acid (13.88 mgL−1), 5-HMF (80.86 mgL−1), furfural (153.54 mgL−1), vanillin (17.17 mgL−1), vanillic acid (85.25 mgL−1), syringaldehyde (41.83 mgL−1), ferulic acid (1.66 mgL−1), and 4-hydroxybenzoic acid (0.85 mgL−1). The study revealed that R. toruloides-1588 and R. toruloides-7191 are promising strains for C5 and C6 sugar utilization from lignocellulosic hydrolysates for lipid production.



中文翻译:

使用C-6和C-5木材水解产物生产拟南芥(Rhodosporidium toruloides)中的脂质的比较研究

球形假单胞菌是一种油脂性酵母,最多可以将其干生物质的70%积累为脂质,并可以使用C5和C6糖作为碳源,这些糖存在于来自不同木质纤维素生物质的水解物中。R. toruloides可以替代林业残留物和获得更高附加值的产品。在这项研究中,五个R. toruloides菌株使用木质纤维素水解产物作为培养基,对酵母菌进行评估以找到最有效的利用C5和C6糖的酵母菌株。所有菌株之间的糖消耗量相似。但是,C6和C5水解产物之间存在差异,最大糖利用率分别为98%和60%(对于C6和C5水解产物)。在所研究的菌株中,在112小时后,在R. toruloides -1588中观察到最高的脂质产量,其中C6水解产物中的脂质为23.33 g / L。而在120小时后,在R. toruloides -7191中观察到C5水解产物的最高脂质产量,其中脂质为14.67 g / L。R. toruloides -1588的脂肪酸主要为油酸,棕榈酸和硬脂酸,而十五碳酸,棕榈酸和十七碳酸为R. toruloides -7191。此外,两种菌株在抑制剂化合物如乙酰丙酸(13.88 mgL -1),5-HMF(80.86 mgL -1),糠醛(153.54 mgL -1),香兰素(17.17 mgL -1)的存在下生长并产生脂质。),香草酸(85.25 mgL -1),丁香醛(41.83 mgL -1),阿魏酸(1.66 mgL -1)和4-羟基苯甲酸(0.85 mgL -1)。该研究显示,R.酵母菌-1588和R.酵母菌-7191是有希望的,从生产油脂木质纤维素水解C5和C6糖利用的菌株。

更新日期:2019-09-27
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