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Exploring the Efficacy of Nile Red in Microplastic Quantification: A Costaining Approach
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2019-09-20 , DOI: 10.1021/acs.estlett.9b00499
Thomas Stanton 1, 2 , Matthew Johnson 1 , Paul Nathanail 3 , Rachel L. Gomes 2 , Teresa Needham 1 , Amanda Burson 1
Affiliation  

The presence of microplastic particles (<5 mm) in the environment has generated considerable concern across public, political, and scientific platforms. However, the diversity of microplastics that persist in the environment poses complex analytical challenges for our understanding of their prevalence. The use of the dye Nile red to quantify microplastics is increasingly common. However, its use in microplastic analysis rarely accounts for its affinity with the breadth of particles that occur in environmental samples. Here, we examine Nile red’s ability to stain a variety of microplastic particles and common natural and anthropogenic particles found in environmental samples. To better constrain microplastic estimates using Nile red, we test the coapplication of a second stain that binds to biological material, 4′,6-diamidino-2-phenylindole (DAPI). We test the potential inflation of microplastic estimates using Nile red alone by applying this costaining approach to samples of drinking water and freshwater. The use of Nile red dye alone resulted in a maximum 100% overestimation of microplastic particles. These findings are of particular significance for the public dissemination of findings from an emotive field of study.

中文翻译:

探索尼罗红在微塑料定量中的功效:一种可可化方法

环境中存在的微塑料颗粒(<5毫米)引起了公共,政治和科学平台的广泛关注。但是,存在于环境中的微塑料的多样性对我们对它们的普遍性的理解提出了复杂的分析挑战。使用染料尼罗红来定量微塑料变得越来越普遍。但是,它在微塑性分析中的使用很少能说明其与环境样品中存在的颗粒宽度的亲和力。在这里,我们研究了尼罗红对环境样本中发现的各种微塑料颗粒以及常见的自然和人为颗粒进行染色的能力。为了更好地限制使用尼罗红的微塑性估计,我们测试了与生物材料4'结合的第二种染色剂的共同应用,6-二mid基-2-苯基吲哚(DAPI)。我们通过对饮用水和淡水样品采用这种共计方法,仅使用尼罗红测试了微塑性估计值的潜在膨胀。单独使用尼罗河红色染料会导致对塑料微粒的最大高估100%。这些发现对于情感研究领域的发现的公开传播具有特别重要的意义。
更新日期:2019-09-21
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