The cyst of the soya bean cyst nematode (SCN; Heterodera glycines), an economically important pathogen of soya beans worldwide, represents a unique microhabitat in soil. The fungi inhabiting cysts may include natural antagonists of the SCN as well as saprotrophs and other opportunists. This study aimed to characterise the entire culturable mycobiome of SCN cysts obtained from a long-term soya bean-corn rotation experiment using ITS fungal barcoding. Fusarium was consistently the most frequently isolated taxon across all sampling time points and crop sequences, followed by Ilyonectria. Among fourteen genera frequently isolated from SCN cysts, five fell within the single family Nectriaceae (Sordariomycetes) and five within the order Pleosporales (Dothideomycetes), suggesting independent evolutionary origins and shared adaptations in these groups towards colonisation of SCN cysts. Six genera (Pochonia, Clonostachys, Fusarium, Neonectria, Alternaria, and Leptosphaeria) varied significantly by crop sequence in at least one year.

大豆囊肿线虫（SCN；杂种甘氨酸）的囊肿是世界范围内重要的大豆病原体，在土壤中具有独特的微生境。居住在真菌中的囊肿可能包括SCN的天然拮抗剂以及腐生菌和其他机会主义者。这项研究旨在表征使用ITS真菌条形码从长期大豆-玉米轮作实验获得的SCN囊肿的整个可培养菌群。在所有采样时间点和农作物序列中，镰刀菌一直是最常被隔离的分类单元，其次是伊利氏菌属。在经常从SCN囊肿中分离出来的14个属中，有5个属于单核霉菌科（Sordariomycetes），有5个属于within骨目（Dothideomycetes），这表明这些物种的独立进化起源和共同适应对SCN囊肿的定殖。六个属（Pochonia，Clonostachys，镰刀，Neonectria，黑斑，和颖）作物序列在至少一年显著变化。