Half adder and half subtractor logic gates based on nicking enzymes†,Molecular Systems Design & Engineering

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Half adder and half subtractor logic gates based on nicking enzymes†
Molecular Systems Design & Engineering ( IF 3.2 ) Pub Date : 2019-09-18 , DOI: 10.1039/c9me00090a
Yunbin Zhao _{1,

2,

3,

4,

5} , Yuan Liu _{5,

6,

7,

8} , Xuedong Zheng _{5,

9,

10,

11} , Bin Wang _{1,

2,

3,

4,

5} , Hui Lv _{1,

2,

3,

4,

5} , Shihua Zhou _{1,

2,

3,

4,

5} , Qiang Zhang _{1,

2,

3,

4,

5} , Xiaopeng Wei _{5,

6,

7,

8}

Affiliation

The excellent specificity and predictability of DNA pairing and its natural ability to interact with other biomolecules make DNA an ideal material for building molecular logic devices (MLDs). However, there are still many challenges in the process of building such devices, including their complex structures, potentially harsh reaction conditions, long reaction times and so on. Thus, the parallel nature of DNA and other biomolecules needs to be developed before MLDs based on DNA can be exploited to a greater extent. In order to solve this problem, herein, we carefully selected two enzymes, Nt.BbvCI and Nb.BtsI, that were both persistent and compatible, and used them to build a nicking enzyme platform. Based on this enzyme platform, we constructed a novel XOR logic gate with flexible internal signaling. Furthermore, AND and INHIBIT logic gates were also modified to use the same enzymes as the XOR logic gate as their inputs. As a result, the algorithm process of half adder and half subtractor was realized by this work. This study provides a new approach for typical DNA-based arithmetic operations and promotes the development of advanced MLDs.

中文翻译：

基于切刻酶的半加法器和半减法器逻辑门†

DNA配对的出色特异性和可预测性以及其与其他生物分子相互作用的天然能力使DNA成为构建分子逻辑器件（MLD）的理想材料。然而，在构建此类装置的过程中仍然存在许多挑战，包括它们的复杂结构，潜在的苛刻反应条件，较长的反应时间等。因此，在可以更广泛地利用基于DNA的MLD之前，需要开发DNA和其他生物分子的平行性质。为了解决这个问题，在这里，我们仔细选择了两个持久且相容的酶Nt.BbvCI和Nb.BtsI，并使用它们来构建切刻酶平台。基于此酶平台，我们构建了具有灵活内部信号的新型XOR逻辑门。此外，还对AND和INHIBIT逻辑门进行了修改，以使用与XOR逻辑门相同的酶作为输入。结果，通过该工作实现了半加法器和半减法器的算法过程。这项研究为典型的基于DNA的算术运算提供了一种新方法，并促进了高级MLD的发展。

更新日期：2019-12-02

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