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The ScRALF3 secreted peptide is involved in sporophyte to gametophyte signalling and affects pollen mitosis I.
Plant Biology ( IF 4.2 ) Pub Date : 2019-10-09 , DOI: 10.1111/plb.13046
A Loubert-Hudon 1 , B D Mazin 1 , É Chevalier 1 , D P Matton 1
Affiliation  

Signalling events through small peptides are essential in multiple aspects of plant reproduction. The ScRALF3 Solanum chacoense Rapid Alkalinization Factor (RALF) peptide was previously shown to regulate multiple aspects of cell-cell communication between the surrounding sporophytic tissue and the female gametophyte during ovule development. We analysed the global expression pattern of ScRALF3 with GUS reporter gene under control of the ScRALF3 promoter and validated it with in situ hybridisation. To better understand the role of ScRALF3 we used three different RNA interference (RNAi) lines that reduced the expression of ScRALF3 during pollen development. Both expression methods showed the presence of ScRALF3 in different tissues, including stigma, style, vascular tissues and during stamen development. Down-regulation of ScRALF3 expression through RNAi showed drastic defects in early stages of pollen development, mainly on the first mitosis. These results suggest that the ScRALF3 secreted peptide regulates the transition from sporogenesis to gametogenesis in both male and female gametophytes.

中文翻译:

ScRALF3分泌的肽参与孢子体配子体信号传导,并影响花粉有丝分裂I。

通过小肽发出的信号事件在植物繁殖的多个方面都是必不可少的。先前显示,ScRALF3茄茄快速碱化因子(RALF)肽在胚珠发育过程中调节周围孢子体组织和雌配子体之间细胞间通讯的多个方面。我们分析了在ScRALF3启动子控制下具有GUS报告基因的ScRALF3的全球表达模式,并通过原位杂交对其进行了验证。为了更好地了解ScRALF3的作用,我们使用了三种不同的RNA干扰(RNAi)品系,它们在花粉发育过程中降低了ScRALF3的表达。两种表达方法均显示ScRALF3存在于不同的组织中,包括柱头,花柱,维管组织以及雄蕊发育过程中。通过RNAi对ScRALF3表达的下调显示出在花粉发育早期的严重缺陷,主要是在第一个有丝分裂上。这些结果表明,ScRALF3分泌的肽调节雄性和雌性配子体中从孢子发生到配子发生的过渡。
更新日期:2019-10-09
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