The biocompatibility of human gingival fibroblasts (HGF) was evaluated in different concentrations of poly(vinyl alcohol) and sodium alginate (PVA/SA) nanofibres (3.5 wt% 4 wt% and 5 wt%). The PVA/SA nanofibres were deposited on the surface of an electrode microchip by using the electrospinning technique. Electrochemical impedance spectroscopy (EIS) was applied to measure the dielectric properties of each system. In order to provide a detailed analysis as well as a right physical interpretation of the EIS results, the data was fitted with an electric equivalent circuit based on the EIS and the microscopic assessments. The results registered three different time constants (TCs) of the PVA/SA scaffold which indicated different layers at different depths of the scaffold. The TCs changed their dielectric properties depending on the PVA/SA concentration. The 4 wt% system showed the highest biocompatibility properties, given that its resistance and electrochemical capacitance show the formation of a mature-stage cell interaction of HGF. The EIS data offers an exhaustive analysis of the biological activity of the cell response in real time to determine its biocompatibility features. Fluorescence analysis demonstrated a heterogeneous growth of the HGF on the PVA/SA scaffold surface.

中文翻译：

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通过使用电化学阻抗谱评估PVA / SA支架与人牙龈成纤维细胞（HGF）的生物相容性。

在不同浓度的聚乙烯醇和海藻酸钠（PVA / SA）纳米纤维（3.5 wt％，4 wt％和5 wt％）中评估了人牙龈成纤维细胞（HGF）的生物相容性。通过使用电纺丝技术将PVA / SA纳米纤维沉积在电极微芯片的表面上。电化学阻抗谱（EIS）用于测量每个系统的介电性能。为了提供详细的分析以及对EIS结果的正确物理解释，数据基于EIS和微观评估数据配备了等效电路。结果记录了PVA / SA支架的三个不同的时间常数（TC），其指示了在支架的不同深度处的不同层。TC根据PVA / SA浓度改变其介电性能。4 wt％的系统显示出最高的生物相容性，因为其电阻和电化学电容显示出HGF的成熟阶段细胞相互作用的形成。EIS数据可实时详尽地分析细胞反应的生物学活性，以确定其生物相容性特征。荧光分析表明，HGF在PVA / SA支架表面上异质生长。