A flexible acetylcholinesterase (AChE) film biosensor, based on a AuNPs-MoS2-reduced graphene oxide/polyimide flexible film (rGO/PI) electrode, has been synthesized for paraoxon detection. In this study, the rGO/PI film acts as the flexible substrate and AuNPs are reduced by monolayer MoS2 under illumination. Transmission electron microscopy revealed that AuNPs are uniformly dispersed on the MoS2-rGO/PI electrode surface with a diameter ~10nm. X-ray photoelectron spectroscopy indicated that a strong binding force exists between reduced AuNPs and monolayer MoS2. The AChE modified AuNPs-MoS2-rGO/PI flexible film biosensor is used to hydrolyze acetylcholine chloride and obtain a large current response at 0.49V by differential pulse voltammetry, demonstrating successful immobilization of AChE. In view of the inhibition of paraoxon on the AChE, under optimal conditions, the AChE/AuNPs-MoS2-rGO/PI film biosensor shows a linear response over a concentration range 0.005-0.150μg/mL, a sensitivity of 4.44 uA/μg/mL, a detection limit of 0.0014μg/mL, acceptable reproducibility and stability to paraoxon. The flexible film biosensor has also proved used for detection of paraoxon in real samples.

中文翻译：

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乙酰胆碱酯酶修饰的AuNPs-MoS2-rGO / PI柔性膜生物传感器：致力于高效制造和在对氧磷检测中的应用。

合成了基于AuNPs-MoS2还原的氧化石墨烯/聚酰亚胺柔性膜（rGO / PI）电极的柔性乙酰胆碱酯酶（AChE）膜生物传感器，用于对氧磷的检测。在这项研究中，rGO / PI薄膜充当柔性基板，并且在照明下通过单层MoS2还原AuNPs。透射电子显微镜显示，AuNPs均匀分散在直径约10nm的MoS2-rGO / PI电极表面上。X射线光电子能谱表明还原的AuNPs和单层MoS2之间存在很强的结合力。AChE修饰的AuNPs-MoS2-rGO / PI柔性膜生物传感器用于水解乙酰胆碱氯化物，并通过差分脉冲伏安法在0.49V时获得大电流响应，证明了AChE的成功固定。鉴于对氧磷对AChE的抑制作用，在最佳条件下，AChE / AuNPs-MoS2-rGO / PI膜生物传感器在0.005-0.150μg/ mL的浓度范围内显示线性响应，灵敏度为4.44 uA /μg/ mL，检测极限为0.0014μg/ mL，对氧磷具有可接受的重现性和稳定性。柔性膜生物传感器还被证明可用于检测实际样品中的对氧磷。