Enhancing the self-buffering capacity is critical in the operation of bufferless microbial fuel cells (BLMFCs). Inorganic carbon (IC) is an ideal endogenous buffer, but its spontaneously accumulated concentration is insufficient to adjust anolyte pH. In this study, BLMFCs were operated with anaerobic sludge to enhance IC accumulation and increase anolyte pH. The accumulated IC concentration during a single running cycle was elevated from 8.3 mM to 12.5 mM, and anolyte pH remained above 7.5. The electric power output was significantly promoted from 332.2 mW·m-2 to 628.1 mW·m-2, and the coulombic efficiency (CE) slightly increased from 16.4% to 19.5%. Geobacter was the electro-active genus in the anode biofilms of the MFCs, and its relative abundance in the KCl-S anode biofilm increased from 0.2% to 5.75%. After continuous operation, the predominant genus of the anaerobic sludge had changed from Flavobacterium to Fusibacter.

中文翻译：

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厌氧污泥对无缓冲单室微生物燃料电池生物发电增强的影响。

在无缓冲微生物燃料电池（BLMFC）的运行中，提高自缓冲能力至关重要。无机碳（IC）是理想的内源缓冲液，但其自发累积的浓度不足以调节阳极电解液的pH。在这项研究中，BLMFCs与厌氧污泥一起使用，以增强IC的积累并增加阳极电解液的pH值。在一个运行周期内，累积的IC浓度从8.3 mM增加到12.5 mM，并且阳极电解液的pH值保持在7.5以上。电力输出从332.2 mW·m-2显着提升到628.1 mW·m-2，库仑效率（CE）从16.4％略微提高到19.5％。地细菌是MFCs阳极生物膜中的电活性属，其在KCl-S阳极生物膜中的相对丰度从0.2％增加到5.75％。连续运行后，