Porcine edema disease (ED) is a toxemia that is caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) and is associated with high mortality. Since ED occurs most frequently during the weaning period, preweaning vaccination of newborn piglets is required. We developed stx2eB-transgenic lettuce as an oral vaccine candidate against ED and examined its protective efficacy using a piglet STEC infection model. Two serially developed Stx2eB-lettuce strains, 2BN containing ingredient Stx2eB constituting a concentration level of 0.53 mg Stx2eB/g of powdered lettuce dry weight (DW) and 2BH containing ingredient Stx2eB constituting a concentration level of 2.3 mg of Stx2eB/g of powdered lettuce DW, were evaluated in three sequential experiments. Taken the results together, oral administration of Stx2eB-lettuce vaccine was suggested to relieve the pathogenic symptoms of ED in piglets challenged with virulent STEC strain. Our data suggested that Stx2eB-lettuce is a promising first oral vaccine candidate against ED.

中文翻译：

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志贺毒素2eB转基因生菜疫苗可有效保护断奶仔猪免受产志贺毒素大肠杆菌感染引起的水肿病。


猪水肿病 (ED) 是一种由产志贺毒素 2e (Stx2e) 的大肠杆菌 (STEC) 肠道感染引起的毒血症，死亡率很高。由于ED最常发生在断奶期，因此需要对新生仔猪进行断奶前疫苗接种。我们开发了 stx2eB 转基因生菜作为针对 ED 的口服候选疫苗，并使用仔猪 STEC 感染模型检查了其保护功效。两个系列开发的Stx2eB-生菜菌株，2BN含有成分Stx2eB，浓度水平为0.53mg Stx2eB/g生菜粉干重(DW)，2BH含有成分Stx2eB，浓度水平为2.3mg Stx2eB/g生菜粉DW ，在三个连续实验中进行评估。综合这些结果，建议口服 Stx2eB 生菜疫苗来缓解受到强毒 STEC 菌株攻击的仔猪的 ED 致病症状。我们的数据表明 Stx2eB-lettuce 是第一个有前途的口服 ED 候选疫苗。