Kaempferol derivatives isolated from Lens culinaris Medik. reduce DNA damage induced by etoposide in peripheral blood mononuclear cells.,Toxicology Research

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Kaempferol derivatives isolated from Lens culinaris Medik. reduce DNA damage induced by etoposide in peripheral blood mononuclear cells.
Toxicology Research ( IF 2.2 ) Pub Date : 2019-09-10 , DOI: 10.1039/c9tx00176j
Magdalena Kluska ₁ , Michał Juszczak ₁ , Daniel Wysokiński ₁ , Jerzy Żuchowski ₂ , Anna Stochmal ₂ , Katarzyna Woźniak ₁

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Bioactive compounds isolated from plants are considered to be attractive candidates for cancer therapy. In this study, we examined the effect of kaempferol, its derivatives, the polyphenol fraction (PF) and an extract (EX) isolated from the aerial parts of Lens culinaris Medik. on DNA damage induced by etoposide in human cells. We also studied the effect of these compounds and their combinations on cell viability. The studies were conducted on HL-60 cells and human peripheral blood mononuclear cells (PBMCs). We used the comet assay in the alkaline version to evaluate DNA damage. To examine cell viability we applied the trypan blue exclusion assay. We demonstrated that kaempferol glycoside derivatives isolated from the aerial parts of Lens culinaris Medik. reduce DNA damage induced by etoposide in PBMCs, but do not have an impact on DNA damage in HL-60 cells. We also showed that kaempferol induces DNA damage in HL-60 cells and leads to an increase of DNA damage provoked by etoposide. Our data suggest that kaempferol derivatives can be further explored as a potential agent protecting normal cells against DNA damage induced by etoposide. Moreover, kaempferol's ability to induce DNA damage in cancer cells and to increase DNA damage caused by etoposide may be useful in designing and improving anticancer therapies.

中文翻译：

从扁豆中提取的山奈酚衍生物。减少依托泊苷诱导的外周血单核细胞DNA损伤。

从植物中分离出的生物活性化合物被认为是癌症治疗的诱人候选物。在这项研究中，我们检查了山萘酚，其衍生物，多酚馏分（PF）和提取物（EX）的提取物，该提取物是从麦地那香豆的地上部分中分离出来的。依托泊苷对人细胞的DNA损伤作用。我们还研究了这些化合物及其组合对细胞活力的影响。这项研究是针对HL-60细胞和人外周血单个核细胞（PBMC）进行的。我们使用了碱性版本的彗星试验来评估DNA损伤。为了检查细胞生存力，我们应用了锥虫蓝排除试验。我们证明了山竹酚糖苷衍生物是从扁豆的地上部分中分离出来的梅迪克减少依托泊甙在PBMC中诱导的DNA损伤，但对HL-60细胞的DNA损伤没有影响。我们还显示山emp酚可诱导HL-60细胞中的DNA损伤，并导致依托泊苷引起的DNA损伤增加。我们的数据表明，山fer酚衍生物可作为保护正常细胞免受依托泊苷诱导的DNA损伤的潜在药物而进一步探索。此外，山奈酚在癌细胞中诱导DNA损伤并增加由依托泊苷引起的DNA损伤的能力可能对设计和改善抗癌治疗有用。

更新日期：2019-09-10

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