OBJECTIVES Two Neisseria meningitidis serogroup B (NmB) vaccines are licensed in the United States. To estimate their potential coverage, we examined the vaccine antigen diversity among meningococcal isolates prior to vaccine licensure. METHODS NmB vaccine antigen genes of invasive isolates collected in the U.S. from 2009 to 2014 were characterized by Sanger or whole-genome sequencing. RESULTS During 2009-2014, the predominant antigen types have remained similar to those reported in 2000-2008 for NmB and 2006-2008 for NmC, NmY, with the emergence of a few new types. FHbp of subfamily B or variant 1 (B/v1) remained prevalent among NmB whereas FHbp of subfamily A or variant 2 and 3 (A/v2-3) were more prevalent among non-NmB. FHbp peptide 1 (B24/1.1) remains the most prevalent type in NmB. Full-length NadA peptide was detected in 26% of isolates, primarily in NmB and NmW. The greatest diversity of NhbA peptides was detected among NmB, with p0005 as the most prevalent type. CONCLUSIONS The prevalence and diversity of the NmB vaccine antigens have remained stable with common antigen types persisting over time. The data collected prior to NmB vaccine licensure provide the baseline to understand the potential impact of NmB vaccines on antigen diversity and strain coverage.

中文翻译：

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在NmB疫苗获得许可之前，2009年至2014年期间，脑膜炎双球菌血清b型（NmB）疫苗抗原在引起脑膜炎双球菌的分离株中的分布。

目的两种乙型脑膜炎奈瑟氏球菌（NmB）疫苗已在美国获得许可。为了估计其潜在的覆盖范围，我们在许可疫苗之前检查了脑膜炎球菌分离株之间的疫苗抗原多样性。方法采用Sanger或全基因组测序技术对2009年至2014年在美国收集的侵袭性分离株的NmB疫苗抗原基因进行表征。结果2009-2014年间，主要抗原类型与2000-2008年报告的NmB和2006-2008年报告的NmC，NmY相似，并出现了一些新类型。在NmB中，亚家族B或变体1（B / v1）的FHbp仍然盛行，而在非NmB中，亚家族A或变体2和3（A / v2-3）的FHbp更为盛行。FHbp肽1（B24 / 1.1）仍然是NmB中最流行的类型。在26％的分离物中检测到全长NadA肽，主要在NmB和NmW中。在NmB中检测到最大的NhbA肽多样性，其中p0005是最普遍的类型。结论NmB疫苗抗原的流行性和多样性一直保持稳定，常见抗原类型随时间推移持续存在。在NmB疫苗获得许可之前收集的数据为了解NmB疫苗对抗原多样性和菌株覆盖范围的潜在影响提供了基准。