Abstract Influenza virus (IFV) detection in the early phase of disease is critical for effective anti-influenza therapy using neuraminidase inhibitors. Sialyloligosaccharide receptors on the surface of respiratory cells are recognized by IFV hemagglutinin (HA) in the infection. Here, we show that agglutination of IFV is detected using poly(glycidyl methacrylate) (PGMA)-coated polystyrene nanoparticles conjugated with a sialic acid-mimic peptide. The azido peptide was immobilized onto the surface of the PGMA-coated nanoparticles by click chemistry. The distribution of particle size, determined by dynamic light scattering, indicated that the peptide-conjugated nanoparticles were agglutinated in the presence of HA and IFV. Nanoparticles conjugated with the receptor-mimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza. This study examines the use of poly(glycidyl methacrylate) (PGMA)-coated polystyrene functionalized with peptides that mimic receptors found on red blood cells for detection of the influenza virus. Although the current method, the hemagglutination inhibition test, utilized by the World Health Organization, is simple, it has some limitations. We found that our peptide dimers attached to beads were efficiently agglutinated, leading us to detect the presence of the influenza virus antigens. We believe that our study makes a significant contribution to the fight against influenza because we provide an alternative method for the important task of influenza surveillance and diagnosis.

中文翻译：

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使用与唾液酸模拟肽偶联的纳米颗粒通过凝集检测流感病毒

摘要 在疾病的早期阶段检测流感病毒 (IFV) 对于使用神经氨酸酶抑制剂进行有效的抗流感治疗至关重要。呼吸道细胞表面的唾液酸寡糖受体在感染中被 IFV 血凝素 (HA) 识别。在这里，我们表明使用与唾液酸模拟肽结合的聚（甲基丙烯酸缩水甘油酯）（PGMA）包被的聚苯乙烯纳米粒子检测 IFV 的凝集。通过点击化学将叠氮肽固定在 PGMA 涂层纳米颗粒的表面。通过动态光散射测定的粒径分布表明，肽缀合的纳米颗粒在 HA 和 IFV 存在下发生凝集。在流感的全球监测和临床诊断中，与受体模拟肽结合的纳米颗粒可能是红细胞的有用替代品。本研究检查了使用聚甲基丙烯酸缩水甘油酯 (PGMA) 包被的聚苯乙烯功能化的肽，模拟红细胞上发现的受体，用于检测流感病毒。世界卫生组织目前采用的血凝抑制试验方法虽然简单，但也有一定的局限性。我们发现附着在珠子上的肽二聚体被有效地凝集，使我们能够检测流感病毒抗原的存在。我们相信我们的研究对抗击流感做出了重大贡献，因为我们为流感监测和诊断的重要任务提供了一种替代方法。