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Reactive oxygen species regulate auxin levels to mediate adventitious root induction in Arabidopsis hypocotyl cuttings.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-11-04 , DOI: 10.1111/jipb.12870 Aixia Huang 1 , Yongshun Wang 1 , Yangyang Liu 1 , Guodong Wang 1 , Xiaoping She 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-11-04 , DOI: 10.1111/jipb.12870 Aixia Huang 1 , Yongshun Wang 1 , Yangyang Liu 1 , Guodong Wang 1 , Xiaoping She 1
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Adventitious root (AR) formation from leafy stem cuttings is critical for breeding of many forest and horticultural species. In addition to the plant hormone auxin, wound‐induced signaling caused by the cutting excision is also essential for AR initiation. Here we found that reactive oxygen species (ROS) are rapidly generated at the excision site as a wound‐induced signal and propagated throughout the hypocotyl cutting after excision of the Arabidopsis (Arabidopsis thaliana) primary root. ROS propagation was not observed in the presence of an NADPH oxidase inhibitor (diphenylene iodonium chloride) or in a knockout mutant of the NADPH oxidase gene respiratory burst oxidase homolog protein D (RBOHD). Respiratory burst oxidase homolog protein D was specifically upregulated in hypocotyl cuttings at 0.5 h post excision (hpe). Together, these data suggest that RBOHD mediates ROS propagation in hypocotyl cuttings. We also found that auxin levels increased significantly in the shoot apex at 5 hpe and at the base of the cutting at 6 hpe; these effects were blocked by treatment with ROS scavengers. Consistent with this, transcript levels of auxin biosynthesis and polar‐transport genes generally increased between 1 to 6 hpe. Collectively, our results suggest that wound‐induced ROS participate in AR induction through regulation of auxin biosynthesis and transport.
中文翻译：
活性氧调节生长素的水平，以调节拟南芥下胚轴插条不定根的诱导。
叶状枝条不定根（AR）的形成对于许多森林和园艺物种的繁殖至关重要。除植物激素植物生长素外，切割切除引起的伤口诱导信号对于AR的引发也很重要。在这里，我们发现，在拟南芥（Arabidopsis thaliana）根部切除后，活性氧（ROS）在伤口切除部位迅速生成为伤口诱导的信号，并在整个下胚轴切口中传播。在存在NADPH氧化酶抑制剂（二亚苯基碘鎓氯化物）或NADPH氧化酶基因呼吸爆发氧化酶同源蛋白D（RBOHD）的敲除突变体中，未观察到ROS传播。切除后0.5 h（hpe），下胚轴插条中的呼吸爆发性氧化酶同源蛋白D特异上调。这些数据加在一起，表明RBOHD介导了下胚轴插条中ROS的传播。我们还发现，在5 hpe时，茎尖的生长素水平显着增加；在6 hpe时，the插的根部的生长素水平显着增加。这些效果被ROS清除剂治疗所阻断。与此相一致，生长素生物合成和极性转运基因的转录水平通常在1至6 hpe之间增加。总体而言，我们的结果表明，伤口诱导的ROS通过调节生长素的生物合成和转运而参与AR的诱导。
更新日期：2019-11-04
中文翻译：
活性氧调节生长素的水平，以调节拟南芥下胚轴插条不定根的诱导。
叶状枝条不定根（AR）的形成对于许多森林和园艺物种的繁殖至关重要。除植物激素植物生长素外，切割切除引起的伤口诱导信号对于AR的引发也很重要。在这里，我们发现，在拟南芥（Arabidopsis thaliana）根部切除后，活性氧（ROS）在伤口切除部位迅速生成为伤口诱导的信号，并在整个下胚轴切口中传播。在存在NADPH氧化酶抑制剂（二亚苯基碘鎓氯化物）或NADPH氧化酶基因呼吸爆发氧化酶同源蛋白D（RBOHD）的敲除突变体中，未观察到ROS传播。切除后0.5 h（hpe），下胚轴插条中的呼吸爆发性氧化酶同源蛋白D特异上调。这些数据加在一起，表明RBOHD介导了下胚轴插条中ROS的传播。我们还发现，在5 hpe时，茎尖的生长素水平显着增加；在6 hpe时，the插的根部的生长素水平显着增加。这些效果被ROS清除剂治疗所阻断。与此相一致，生长素生物合成和极性转运基因的转录水平通常在1至6 hpe之间增加。总体而言，我们的结果表明，伤口诱导的ROS通过调节生长素的生物合成和转运而参与AR的诱导。
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