Methylobacterium sp. is isolated from water distribution systems and has been linked in the biofilms of the systems with a lower presence of Mycobacterium avium. In this study we aimed to determine the in vitro activity of Methylobacterium sp. in the development of rapidly growing mycobacteria (RGM) biofilms. Methylobacterium sp. CECT 7805 was added as a suspension of living bacteria (LB), an autoclaved suspension (AS), and an extract obtained after sonication (ES) at different times (24, 48, and 72 h), to preformed biofilms of Mycobacterium abscessus DSM 44196, Mycobacterium chelonae ATCC 19235, and Mycobacterium fortuitum ATCC 6841, using a 96 h control of each species. The biofilms were analyzed by confocal laser scanning microscopy and by the Calgary biofilm device using the plates MBEC^TM Biofilm Inoculator. A statistically significant reduction in the thickness and covered surface was observed in all mycobacterial biofilms with all forms of Methylobacterium sp. A statistically significant increase in the autofluorescence was observed in M. abscessus biofilms but not in other biofilms. The increased percentage of dead mycobacteria was statistically significant in all cases. The reduced log CFU (colony-forming units)/peg recount was statistically significant in M. chelonae biofilms after treatment with AS and ES, but in M. fortuitum biofilms the recount decreased only with AS. M. abscessus biofilms were always significantly reduced with AS at 72 h and with ES. Methylobacterium sp. could inhibit RGM biofilm formation. Living cells of Methylobacterium sp. were not necessary to inhibit the growth of a preformed biofilm. M. chelonae biofilms were the most greatly reduced.

甲基杆菌属 从水分配系统中分离出来，并已与鸟分枝杆菌含量较低的系统生物膜相连。在这项研究中，我们旨在确定Methylobacterium sp。的体外活性。在快速增长的分枝杆菌（RGM）生物膜的开发中。甲基杆菌属 将CECT 7805作为活菌悬液（LB），高压灭菌悬液（AS）和在不同时间（24、48和72 h）超声处理（ES）后获得的提取物添加到脓肿分枝杆菌DSM的生物膜中44196，chelonae chelonae ATCC 19235和fortuitum分支杆菌ATCC 6841，使用每个物种96小时的控制时间。通过共聚焦激光扫描显微镜和使用平板MBEC ^TM 生物膜接种器的卡尔加里生物膜装置分析生物膜。在具有所有形式的甲基杆菌属的所有分枝杆菌生物膜中，观察到厚度和覆盖表面的统计学显着减少。在脓肿分支杆菌生物膜中观察到自发荧光的统计学显着增加，而在其他生物膜中则未观察到。在所有情况下，死去的分枝杆菌百分比的增加在统计学上都是显着的。在用AS和ES处理后，在chelonae生物膜中，log CFU（菌落形成单位）/ peg计数的减少具有统计学意义，但在福特分枝杆菌生物膜的计数仅在AS时下降。脓肿分枝杆菌的生物膜总是在72 h出现AS和ES时明显减少。甲基杆菌属 可以抑制RGM生物膜的形成。甲基杆菌属的活细胞。不需要抑制预制生物膜的生长。M. chelonae生物膜被最大程度地减少。