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Composite clinoptilolite/PCL-PEG-PCL scaffolds for bone regeneration: In vitro and in vivo evaluation.
Journal of Tissue Engineering and Regenerative Medicine ( IF 3.1 ) Pub Date : 2019-12-02 , DOI: 10.1002/term.2938
Ahmet Engin Pazarçeviren 1 , Tayfun Dikmen 2 , Korhan Altunbaş 2 , Volkan Yaprakçı 3 , Özge Erdemli 4 , Dilek Keskin 1, 5 , Ayşen Tezcaner 1, 5
Affiliation  

In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds were analyzed for compressive strength, biodegradation, biocompatibility, and induction of osteogenic differentiation in vitro. CLN inclusion in PC-10 (10% w/w) and PC-20 (20% w/w) scaffolds revealed 54.7% and 53.4% porosity, higher dry (0.62 and 0.76 MPa), and wet (0.37 and 0.45 MPa) compressive strength, greater cellular adhesion, alkaline phosphatase activity (2.20 and 2.82 mg/gDNA /min), and intracellular calcium concentration (122.44 and 243.24 g Ca/mgDNA ). The scaffolds were evaluated in a unicortical bone defect at anterior aspect of proximal tibia of adult rabbits 4 and 8 weeks postimplantation. Similar to in vitro results, CLN-containing scaffolds led to efficient regeneration of bone in a dose-dependent manner. PC-20 demonstrated highest quality of bone union, cortex development, and bone-scaffold interaction at the defect site. Therefore, higher CLN content in PC-20 permitted robust remodeling whereas pure PCEC (PC-0) scaffolds displayed fibrous tissue formation. Consequently, CLN was proven to be a potent reinforcement in terms of promoting mechanical, physical, and biological properties of polymer-based scaffolds in a more economical, easy-to-handle, and reproducible approach.

中文翻译:

用于骨骼再生的复合斜发沸石/ PCL-PEG-PCL支架:体外和体内评估。

在这项研究中,斜发沸石(CLN)被用作骨组织工程中基于聚合物的复合支架的增强材料,并首次在体内进行了评估。用改良的颗粒浸出/压缩成型技术制造具有高度多孔性,机械稳定性和成骨性的CLN / PCL-PEG-PCL(CLN / PCEC)支架,并改变CLN含量。我们假设复合支架中的CLN增强将改善骨骼再生并促进修复。因此,在体外分析了支架的抗压强度,生物降解,生物相容性和成骨分化的诱导。在PC-10(10%w / w)和PC-20(20%w / w)支架中包含CLN时显示出54.7%和53.4%的孔隙率,较高的干度(0.62和0.76 MPa)和湿润的(0.37和0.45 MPa)抗压强度,更大的细胞粘附力,碱性磷酸酶活性(2.20和2.82 mg / gDNA / min)和细胞内钙浓度(122.44和243.24 g Ca / mgDNA)。在植入后4周和8周,评估成年兔胫骨近端前侧单皮质骨缺损的支架。与体外结果相似,含CLN的支架以剂量依赖性方式导致骨的有效再生。PC-20在缺损部位表现出最高的骨结合,皮质发育和骨-支架相互作用质量。因此,PC-20中较高的CLN含量可实现强大的重塑,而纯PCEC(PC-0)支架则显示出纤维组织的形成。因此,事实证明,CLN可以有效地增强聚合物基脚手架的机械,物理和生物学特性,并且更经济,更易于操作,
更新日期:2019-12-02
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