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A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-serogroup Protection in Mice Against Vaginal Shedding and Infertility.
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2020-01-02 , DOI: 10.1093/infdis/jiz438
Delia F Tifrea 1 , Sukumar Pal 1 , Luis M de la Maza 1
Affiliation  

BACKGROUND Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. Here, we determined the ability of a C. trachomatis recombinant major outer membrane protein (rMOMP) vaccine to elicit cross-serogroup protection. METHODS Female C3H/HeN mice were vaccinated by mucosal and systemic routes with C. trachomatis serovar D (UW-3/Cx) rMOMP and challenged in the ovarian bursa with serovars D (UW-3/Cx), D (UCI-96/Cx), E (IOL-43), or F (N.I.1). CpG-1826 and Montanide ISA 720 were used as adjuvants. RESULTS Immune responses following vaccination were more robust against the most closely related serovars. Following a genital challenge (as determined by number of mice with positive vaginal cultures, number of positive cultures, number of inclusion forming units recovered, and number of days with positive cultures) mice challenged with C. trachomatis serovars of the same complex were protected but not those challenged with serovar F (N.I.1) from a different subcomplex. Females were caged with male mice. Based on fertility rates, number of embryos, and hydrosalpinx formation, vaccinated mice were protected against challenges with serovars D (UW-3/Cx), D (UCI-96/Cx), and E (IOL-43) but not F (N.I.1). CONCLUSIONS This is the first subunit vaccine shown to protect mice against infection, pathology, and infertility caused by different C. trachomatis serovars.

中文翻译:

重组沙眼衣原体MOMP疫苗对小鼠的阴道脱落和不育症具有交叉血清群保护作用。

背景技术沙眼衣原体是全世界最常见的性传播细菌病原体。在这里,我们确定了沙眼衣原体重组主要外膜蛋白(rMOMP)疫苗引起跨血清群保护的能力。方法用沙眼衣原体血清型D(UW-3 / Cx)rMOMP通过粘膜和全身途径给雌性C3H / HeN小鼠接种疫苗,并在血清囊中用血清型D(UW-3 / Cx),D(UCI-96 / Cx),E(IOL-43)或F(NI1)。CpG-1826和Montanide ISA 720用作佐剂。结果接种疫苗后的免疫反应对最密切相关的血清型更有效。进行生殖器攻击后(由阴道培养阳性的小鼠数,阳性培养物的数量,回收的包涵体形成单位的数量确定,阳性培养的天数),用相同复合物的沙眼衣原体血清型攻击的小鼠受到保护,但用不同亚复合物的血清型F(NI1)攻击的小鼠不受保护。将雌性与雄性小鼠关在一起。根据受精率,胚胎数和输卵管积水的形成,接种疫苗的小鼠可免受血清D(UW-3 / Cx),D(UCI-96 / Cx)和E(IOL-43)的攻击,但不受F( NI1)。结论这是第一个被证明可以保护小鼠免受不同沙眼衣原体血清型引起的感染,病理学和不育的亚单位疫苗。接种疫苗的小鼠受到血清D(UW-3 / Cx),D(UCI-96 / Cx)和E(IOL-43)的攻击,但不受F(NI1)的攻击。结论这是第一个被证明可以保护小鼠免受不同沙眼衣原体血清型引起的感染,病理学和不育的亚单位疫苗。接种疫苗的小鼠受到血清D(UW-3 / Cx),D(UCI-96 / Cx)和E(IOL-43)的攻击,但不受F(NI1)的攻击。结论这是第一个被证明可以保护小鼠免受不同沙眼衣原体血清型引起的感染,病理学和不育的亚单位疫苗。
更新日期:2019-12-30
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