Arabidopsis DXO1 possesses deNADding and exonuclease activities and its mutation affects defense-related and photosynthetic gene expression.,Journal of Integrative Plant Biology

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Arabidopsis DXO1 possesses deNADding and exonuclease activities and its mutation affects defense-related and photosynthetic gene expression.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-11-07 , DOI: 10.1111/jipb.12867
Shuying Pan ₁ , Kai-En Li ₁ , Wei Huang ₂ , Huan Zhong ₁ , Huihui Wu ₃ , Yuan Wang ₄ , He Zhang ₁ , Zongwei Cai ₂ , Hongwei Guo ₃ , Xuemei Chen ₄ , Yiji Xia _{1,

2,

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Affiliation

RNA capping and decapping tightly coordinate with transcription, translation, and RNA decay to regulate gene expression. Proteins in the DXO/Rai1 family have been implicated in mRNA decapping and decay, and mammalian DXO was recently found to also function as a decapping enzyme for NAD⁺‐capped RNAs (NAD‐RNA). The Arabidopsis genome contains a single gene encoding a DXO/Rai1 protein, AtDXO1. Here we show that AtDXO1 possesses both NAD‐RNA decapping activity and 5ʹ‐3ʹ exonuclease activity but does not hydrolyze the m⁷G cap. The atdxo1 mutation increased the stability of NAD‐RNAs and led to pleiotropic phenotypes, including severe growth retardation, pale color, and multiple developmental defects. Transcriptome profiling analysis showed that the atdxo1 mutation resulted in upregulation of defense‐related genes but downregulation of photosynthesis‐related genes. The autoimmunity phenotype of the mutant could be suppressed by either eds1 or npr1 mutation. However, the various phenotypes associated with the atdxo1 mutant could be complemented by an enzymatically inactive AtDXO1. The atdxo1 mutation apparently enhances post‐transcriptional gene silencing by elevating levels of siRNAs. Our study indicates that AtDXO1 regulates gene expression in various biological and physiological processes through its pleiotropic molecular functions in mediating RNA processing and decay.

中文翻译：

拟南芥DXO1具有deNADding和核酸外切酶活性，其突变影响防御相关和光合基因的表达。

RNA加帽和开盖与转录，翻译和RNA衰变紧密协调，以调节基因表达。DXO / Rai1家族中的蛋白质与mRNA的去壳和衰变有关，最近发现哺乳动物DXO还可作为NAD ⁺帽RNA（NAD-RNA）的去壳酶。在拟南芥基因组中包含编码DXO / Rai1蛋白，AtDXO1单个基因。在这里，我们显示AtDXO1既具有NAD-RNA解封活性，又具有5ʹ-3ʹ核酸外切酶活性，但不水解m ⁷ G帽。该atdxo1突变增加了NAD-RNA的稳定性并导致多效性表型，包括严重的生长迟缓，肤色苍白和多种发育缺陷。转录组谱分析表明，atdxo1突变导致防御相关基因的上调，而光合作用相关基因的下调。该突变体的自身免疫表型可以被eds1或npr1突变抑制。但是，与atdxo1突变体相关的各种表型可以被无酶活性的AtDXO1所补充。该atdxo1突变显然通过提高siRNA的水平来增强转录后基因的沉默。我们的研究表明，AtDXO1通过介导RNA加工和衰变的多效分子功能来调节各种生物学和生理过程中的基因表达。

更新日期：2019-11-07

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