In this article, we investigated the sequence specific interaction of single (ssDNA) and double stranded (dsDNA) with silver ions (Ag⁺) with electrochemical methods. We, for the first time, examined the effect of base sequences, base content and physiochemical properties of different DNA sequences on interaction with Ag⁺ in detail. We used different base contents to show how the composition of nucleic acid influences the electrochemical signals. We first immobilized ssDNA probes on bare graphite electrodes. Then, we showed the sequence effect on oxidation signals of AgDNA complex by sensing Ag⁺ to the probe coated surfaces to interact with different ssDNA sequences. Furthermore, we investigated the effect of Ag⁺ on dsDNA. We measured the oxidation signals obtained from Ag⁺‐ssDNA and Ag⁺‐dsDNA complex at approximately 0.2 V and 1.0 V (vs Ag/AgCl), respectively with Differential Pulse Voltammetry (DPV). We showed that the oxidation signals of the AgDNA complex obtained from dsDNA‐modified electrodes is higher than the electrodes modified with ssDNA. More importantly, we showed that Ag⁺‐ssDNA and Ag⁺ ion‐dsDNA exhibit different electrochemical behaviors.

中文翻译：

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金属离子对特定DNA序列和DNA杂交的影响研究

在本文中，我们通过电化学方法研究了单离子（ssDNA）和双链（dsDNA）与银离子（Ag ⁺）的序列特异性相互作用。我们首次详细研究了碱基序列，碱基含量和不同DNA序列的理化性质对与Ag ⁺相互作用的影响。我们使用了不同的碱基含量来显示核酸的组成如何影响电化学信号。我们首先将ssDNA探针固定在裸露的石墨电极上。然后，我们通过感应Ag ⁺到探针包被的表面与不同的ssDNA序列相互作用，显示了序列对AgDNA复合物氧化信号的影响。此外，我们研究了银⁺的作用在dsDNA上。我们用差动脉冲伏安法（DPV）分别测量了从Ag ⁺ -ssDNA和Ag ⁺ -dsDNA复合物获得的氧化信号，电压分别约为0.2 V和1.0 V（vs Ag / AgCl）。我们表明，从dsDNA修饰的电极获得的AgDNA复合物的氧化信号高于用ssDNA修饰的电极。更重要的是，我们表明Ag ⁺ -ssDNA和Ag ⁺ ion-dsDNA表现出不同的电化学行为。