Identification of plant genes putatively involved in the perception of fungal ergosterol-squalene.,Journal of Integrative Plant Biology

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Identification of plant genes putatively involved in the perception of fungal ergosterol-squalene.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-12-30 , DOI: 10.1111/jipb.12862
Laura Lindo _{1,

2} , Rosa E Cardoza _{1,

2} , Alicia Lorenzana ₂ , Pedro A Casquero ₂ , Santiago Gutiérrez _{1,

2}

Affiliation

Trichoderma biocontrol strains establish a complex network of interactions with plants, in which diverse fungal molecules are involved in the recognition of these fungi as nonpathogenic organisms. These molecules act as microbial‐associated molecular patterns that trigger plant responses. Previous studies have reported the importance of ergosterol produced by Trichoderma spp. for the ability of these fungi to induce plant growth and defenses. In addition, squalene, a sterol biosynthetic intermediate, seems to play an important role in these interactions. Here, we analyzed the effect of different concentrations of ergosterol and squalene on tomato (Solanum lycopersicum) growth and on the transcription level of defense‐ and growth‐related genes. We used an RNA‐seq strategy to identify several tomato genes encoding predicted pattern recognition receptor proteins or WRKY transcription factors, both of which are putatively involved in the perception and response to ergosterol and squalene. Finally, an analysis of Arabidopsis thaliana mutants lacking the genes homologous to these tomato candidates led to the identification of a WRKY40 transcription factor that negatively regulates salicylic acid‐related genes and positively regulates ethylene‐ and jasmonate‐related genes in the presence of ergosterol and squalene.

中文翻译：

鉴定与真菌麦角固醇-角鲨烯有关的植物基因的鉴定。

木霉生物防治菌株建立了与植物相互作用的复杂网络，其中多种真菌分子参与了将这些真菌识别为非致病性生物。这些分子充当与微生物相关的分子模式，触发植物反应。先前的研究报道了木霉属植物产生的麦角固醇的重要性。这些真菌诱导植物生长和防御的能力。此外，角鲨烯（一种固醇生物合成中间体）似乎在这些相互作用中起着重要作用。在此，我们分析了不同浓度的麦角固醇和角鲨烯对番茄的作用（番茄）生长以及防御和生长相关基因的转录水平。我们使用RNA-seq策略鉴定了几个编码预测的模式识别受体蛋白或WRKY转录因子的番茄基因，这两个基因均可能参与了麦角固醇和角鲨烯的感知和反应。最后，对缺少与这些番茄候选基因同源的基因的拟南芥突变体的分析导致鉴定出WRKY40转录因子，该转录因子在麦角固醇和角鲨烯存在的情况下负调控水杨酸相关基因并正调控乙烯和茉莉酸酯相关基因。。

更新日期：2019-12-30

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