Beyond the paradigm of nanomechanical measurements on cells using AFM: an automated methodology to rapidly analyse thousands of cells†,Nanoscale Horizons

当前位置： X-MOL 学术 › Nanoscale Horiz. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Beyond the paradigm of nanomechanical measurements on cells using AFM: an automated methodology to rapidly analyse thousands of cells†
Nanoscale Horizons ( IF 8.0 ) Pub Date : 2019-08-15 , DOI: 10.1039/c9nh00438f
S. Proa-Coronado _{1,

2,

3,

4,

5} , C. Séverac _{6,

7,

8,

9,

10} , A. Martinez-Rivas _{1,

2,

3,

4,

5} , E. Dague _{7,

8,

9,

10,

11}

Affiliation

Nanomechanical properties of cells could be considered as cellular biomarkers. The main method used to access the mechanical properties is based on nanoindentation measurements, performed with an operator manipulated Atomic Force Microscope (AFM) which is time-consuming and expensive. This is one of the reasons that prevent the transfer of AFM technology into clinical laboratories. In this paper we report a methodology which includes an algorithm (transferred to a script, executed on a commercial AFM) able to automatically move the tip onto a single cell and through several cells to record force curves combined with a smart strategy of cell immobilization. Cells are placed into microwells of a microstructured polydimethylsiloxane (PDMS) stamp. Inside a classical 100 × 100 μm² AFM field, 100 cells can be immobilized. In an optimal configuration we were able to measure, within 4 h, a population of 900 Candida albicans cells both native and caspofungin treated, which represents an unprecedented performance. We discovered that the population is heterogeneous and can be divided, on the basis of nanomechanical properties, into 2 subgroups.

中文翻译：

超越了使用原子力显微镜对细胞进行纳米力学测量的范式：一种可快速分析成千上万细胞的自动化方法†

细胞的纳米力学性质可以被认为是细胞生物标志物。用于获得机械性能的主要方法是基于纳米压痕测量，这是由操作员操纵的原子力显微镜（AFM）进行的，既耗时又昂贵。这是阻止将AFM技术转移到临床实验室的原因之一。在本文中，我们报告了一种方法，该方法包括一种算法（转移到脚本中，在商用AFM上执行），该算法能够将尖端自动移动到单个单元格上并通过多个单元格来记录力曲线，并结合了智能化的单元格固定策略。将细胞放入微结构化聚二甲基硅氧烷（PDMS）压模的微孔中。内部的古典100×100微米²在AFM领域，可以固定100个细胞。在最佳配置下，我们能够在4小时内测量900个经过处理的天然念珠菌和经卡泊芬净治疗的白色念珠菌细胞的数量，这代表了前所未有的性能。我们发现该种群是异质的，并且可以根据纳米力学特性分为2个亚组。

更新日期：2019-12-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11