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The synergistic effects of slightly acidic electrolyzed water and UV-C light on the inactivation of Salmonella enteritidis on contaminated eggshells.
Poultry Science ( IF 3.8 ) Pub Date : 2019-08-08 , DOI: 10.3382/ps/pez454
Sh Bing 1 , Y T Zang 1 , Y J Li 1 , D Q Shu 1
Affiliation  

Salmonella enteritidis (S. enteritidis) infection has been recognized as one of the most common bacterial causes of human gastroenteritis worldwide and is closely associated with eggs. Slightly acidic electrolyzed water (SAEW) is an emerging environmentally friendly technology for disinfecting eggshell surfaces to remove dirt and pathogenic microorganisms. However, the efficiency of SAEW could be affected by the presence of manure. UV-based advanced oxidation processes have been studied to improve the microorganism's inactivation effect of disinfection. Therefore, in this study, the synergistic bactericidal efficacy of SAEW and UV-C light (ultraviolet lamp, λ = 254 nm) for inactivation of S. enteritidis on artificially inoculated eggshells with or without manure was evaluated, and the bactericidal efficacy of different combination treatments of SAEW and UV-C light was compared. Without manure interference, complete inactivation (reduction of 6.54 log10 CFU/g) of S. enteritidis on the surface of eggshells was achieved following a 4-min treatment with SAEW+UV at an available chlorine concentration (ACC) of 20 mg/L. In the presence of manure, a 3.02 log reduction was achieved following a 4-min treatment with SAEW+UV at an ACC of 30 mg/L. Simultaneous treatment with SAEW and UV light exhibits higher bactericidal activity for eggshells than other combination process methods with UV and SAEW. The results suggest that the combined treatment of SAEW+UV is a novel method to enhance the microbial safety of eggshells.

中文翻译:

微酸性电解水和UV-C光对污染蛋壳肠炎沙门氏菌灭活的协同作用。

肠炎沙门氏菌 (S. enteritidis) 感染已被公认为全球人类胃肠炎最常见的细菌原因之一,并且与鸡蛋密切相关。微酸性电解水 (SAEW) 是一种新兴的环保技术,用于对蛋壳表面进行消毒,以去除污垢和病原微生物。然而,粪便的存在可能会影响 SAEW 的效率。已经研究了基于紫外线的高级氧化工艺以提高微生物的消毒灭活效果。因此,在本研究中,评估了 SAEW 和 UV-C 光(紫外线灯,λ = 254 nm)对人工接种蛋壳上的肠炎沙门氏菌灭活的协同杀菌效果,无论有无粪便,并比较了SAEW和UV-C光不同组合处理的杀菌效果。在没有粪便干扰的情况下,在有效氯浓度 (ACC) 为 20 mg/L 的情况下,用 SAEW+UV 处理 4 分钟后,蛋壳表面的肠炎沙门氏菌完全灭活(减少 6.54 log10 CFU/g)。在粪便存在的情况下,在 30 mg/L 的 ACC 下用 SAEW+UV 处理 4 分钟后,减少了 3.02 log。SAEW 和 UV 光同时处理对蛋壳的杀菌活性高于其他 UV 和 SAEW 组合处理方法。结果表明,SAEW+UV联合处理是一种提高蛋壳微生物安全性的新方法。在有效氯浓度 (ACC) 为 20 mg/L 的情况下,用 SAEW+UV 处理 4 分钟后,蛋壳表面的肠炎沙门氏菌达到了 54 log10 CFU/g)。在粪便存在的情况下,在 30 mg/L 的 ACC 下用 SAEW+UV 处理 4 分钟后,减少了 3.02 log。SAEW 和 UV 光同时处理对蛋壳的杀菌活性高于其他 UV 和 SAEW 组合处理方法。结果表明,SAEW+UV联合处理是一种提高蛋壳微生物安全性的新方法。在有效氯浓度 (ACC) 为 20 mg/L 的情况下,用 SAEW+UV 处理 4 分钟后,蛋壳表面的肠炎沙门氏菌达到了 54 log10 CFU/g)。在粪便存在的情况下,在 30 mg/L 的 ACC 下用 SAEW+UV 处理 4 分钟后,减少了 3.02 log。SAEW 和 UV 光同时处理对蛋壳的杀菌活性高于其他 UV 和 SAEW 组合处理方法。结果表明,SAEW+UV联合处理是一种提高蛋壳微生物安全性的新方法。SAEW 和 UV 光同时处理对蛋壳的杀菌活性高于其他 UV 和 SAEW 组合处理方法。结果表明,SAEW+UV联合处理是一种提高蛋壳微生物安全性的新方法。SAEW 和 UV 光同时处理对蛋壳的杀菌活性高于其他 UV 和 SAEW 组合处理方法。结果表明,SAEW+UV联合处理是一种提高蛋壳微生物安全性的新方法。
更新日期:2020-04-17
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