The objective of this study was to evaluate the effects of using encapsulated nitrate product (ENP) replacing soybean meal in diets differing in concentrate to forage ratio on ruminal fermentation and methane production in vitro using a semi-automatic gas production technique. Eight treatments were used in a randomized complete design with a 2 × 4 factorial arrangement: two diet (20C:80F and 80C:20F concentrate to forage ratio) and four levels of ENP addition (0%, 1.5%, 3.0%, and 4.5% of DM) replacing soybean meal. There was a diet × ENP interaction (p = 0.02) for methane production. According to ENP addition, diets with 80C:20F showed more intense reduction on methane production that 20C:80F. A negative linear effect was observed for propionate production with ENP addition in diet with 80C:20F and to the relative abundance of methanogens Archaea, in both diet. The replacement of soybean meal by ENP in levels up to 3% of DM inhibited methane production due to a reduction in the methanogens community without affecting the organic matter degradability. However, ENP at 4.5% of DM level affected fiber degradability, abundance of cellulolytic bacteria, and propionic acid production, indicating that this level of inclusion is not recommended for ruminant production.

中文翻译：

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胶囊化硝酸盐替代豆粕会改变精料与饲料比例不同的日粮中的体外瘤胃发酵和甲烷产量。


本研究的目的是利用半自动产气技术，评估在不同精料与饲料比例的日粮中使用胶囊化硝酸盐产品（ENP）替代豆粕对瘤胃发酵和体外甲烷产生的影响。采用 2 × 4 因子排列的随机完整设计中使用了八种处理：两种日粮（20C:80F 和 80C:20F 浓缩物与草料的比例）和四个 ENP 添加水平（0%、1.5%、3.0% 和 4.5） % DM) 替代豆粕。甲烷产量存在饮食 × ENP 相互作用 (p = 0.02)。根据 ENP 的补充，80C:20F 的饮食比 20C:80F 的饮食更能显着减少甲烷产量。在 80°C:20°F 的饮食中添加 ENP 后，观察到丙酸产量的负线性效应以及两种饮食中产甲烷菌古生菌的相对丰度。用 ENP 替代豆粕，其含量高达 DM 的 3%，由于产甲烷菌群落的减少，抑制了甲烷的产生，但不影响有机物的降解性。然而，DM 水平的 4.5% 的 ENP 会影响纤维的降解性、纤维素分解细菌的丰度和丙酸的生产，表明不建议将这种含量水平用于反刍动物生产。