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Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C.
Animal Science Journal ( IF 1.7 ) Pub Date : 2019-08-05 , DOI: 10.1111/asj.13281
Tian-Yu Feng 1 , Fa Ren 1 , Qian Fang 2 , Gui-Chao Dai 1 , Yu Li 1 , Qian Li 1 , Hua-Ming Xi 1 , Hao Li 1 , Yang-Yi Hao 3 , Jian-Hong Hu 1
Affiliation  

Sulfanilamide (SA) is an effective broad-spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.

中文翻译:

磺胺对17°C储液期间公猪精子质量,细菌组成和繁殖力的影响。

磺胺(SA)是人类和兽医学中一种有效的广谱抗菌剂。这项研究的目的是评估在17°C的液体储存过程中SA对公猪精子质量的影响,并确定SA的最佳浓度及其对细菌生长,微生物组成和母体繁殖力的影响。将公猪射精用含有不同浓度SA的碱性增量剂稀释,并在17°C的恒温箱中保存6天。每天测量精子活力,质膜完整性和顶体完整性。结果表明,当SA浓度为0.02 g / L时,精子质量参数显着高于所有其他治疗组(p <.05)。我们还监视了细菌的生长,并比较了0之间的微生物种类差异。02 g / L SA组和对照组采用16S rDNA测序。结果表明,0.02 g / L SA组中的某些细菌(例如葡萄球菌和假单胞菌)明显低于对照组(p <.05)。另外,采用保留的精液进行人工授精,结果表明0.02 g / L的SA组产仔数更高,其妊娠率为92.5%。
更新日期:2019-08-05
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