Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.,Cell Chemical Biology

当前位置： X-MOL 学术 › Cell Chem. Bio. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.
Cell Chemical Biology ( IF 6.6 ) Pub Date : 2019-08-01 , DOI: 10.1016/j.chembiol.2019.07.007
Daniela Boassa ₁ , Sakina P Lemieux ₂ , Varda Lev-Ram ₂ , Junru Hu ₁ , Qing Xiong ₂ , Sebastien Phan ₁ , Mason Mackey ₁ , Ranjan Ramachandra ₁ , Ryan Emily Peace ₃ , Stephen R Adams ₂ , Mark H Ellisman ₄ , John T Ngo ₃

Affiliation

A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as "multicolor" electron microscopy (EM) via the photooxidation of 3-3'-diaminobenzidine and its derivatives.

中文翻译：

Split-miniSOG，用于通过相关的光和电子显微镜在空间上检测细胞内蛋白质与蛋白质的相互作用。

通过将miniSOG分为两部分，建立了用于检测和定位细胞内蛋白质-蛋白质相互作用（PPI）的蛋白质片段互补测定法（PCA），miniSOG是一种从拟南芥光蛋白的光，氧，电压（LOV）-2域衍生的荧光黄素蛋白。当通过相互作用的蛋白质聚集在一起时，这些片段会重新构建一个功能性报告基因，从而使标记的蛋白质复合物可以通过荧光显微镜（LM）可视化，然后通过3-氧化脱氧而通过标准以及“多色”电子显微镜（EM）可视化。 3'-二氨基联苯胺及其衍生物。

更新日期：2019-11-09

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11