Biocatalysts based on immobilized enzymes received considerable attention due to important applications in syntheses of value-added chemicals, pharmaceuticals and drug intermediates with great catalytic efficiency and high yields of target molecules. The important advantages of such biocatalysts are enhanced stability in tolerant pH and temperature range, separation from reaction solutions, stability in repeated use, and so on. In this review, we discuss recent findings in biocatalyst design, in particular, types of promising supports, the biocatalyst surface modification, and incorporation of magnetic nanoparticles for facilitated magnetic recovery. Furthermore, we highlight the development of multienzyme and enzyme/nanoparticle catalysts for cascade reactions, which are carried out in a one-pot process and allow elimination of isolation and purification of intermediates.

基于固定化酶的生物催化剂由于在具有高催化效率和高目标分子产率的增值化学品，药物和药物中间体的合成中的重要应用而受到了广泛的关注。这种生物催化剂的重要优点是在耐受的pH和温度范围内的稳定性增强，与反应溶液的分离，重复使用的稳定性等。在这篇综述中，我们讨论了生物催化剂设计中的最新发现，特别是有前途的载体的类型，生物催化剂的表面改性以及磁性纳米颗粒的掺入，以促进磁性回收。此外，我们重点介绍了用于级联反应的多酶和酶/纳米颗粒催化剂的开发，