Despite being linked to the fundamental processes of chromosome segregation and offspring diversification, meiotic recombination rates vary within and between species. Recent years have seen progress in quantifying recombination rate evolution across multiple temporal and genomic scales. Nevertheless, the level of variation in recombination rate within wild populations—a key determinant of evolution in this trait—remains poorly documented on the genomic scale. To address this notable gap, we used immunofluorescent cytology to quantify genome-wide recombination rates in males from a wild population of the white-footed mouse, Peromyscus leucopus. For comparison, we measured recombination rates in a second population of male P. leucopus raised in the laboratory and in male deer mice from the subspecies Peromyscus maniculatus bairdii. Although we found differences between individuals in the genome-wide recombination rate, levels of variation were low—within populations, between populations, and between species. Quantification of synaptonemal complex length and crossover positions along chromosome 1 using a novel automated approach also revealed conservation in broad-scale crossover patterning, including strong crossover interference. We propose stabilizing selection targeting recombination or correlated processes as the explanation for these patterns.

中文翻译：

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白足小鼠全基因组重组率的保守

尽管与染色体分离和后代多样化的基本过程有关，减数分裂重组率在物种内部和物种之间有所不同。近年来，在跨多个时间和基因组尺度量化重组率演变方面取得了进展。然而，野生种群内重组率的变异水平——这一性状进化的关键决定因素——在基因组规模上的记录仍然很少。为了解决这个显着的差距，我们使用免疫荧光细胞学来量化来自白足小鼠野生种群的雄性的全基因组重组率，Peromyscus leucopus。为了比较，我们测量了在实验室饲养的第二个雄性 P. leucopus 种群和来自 Peromyscus maniculatus bairdii 亚种的雄性鹿小鼠的重组率。尽管我们发现个体之间的全基因组重组率存在差异，但变异水平很低——在种群内、种群之间和物种之间。使用一种新的自动化方法对 1 号染色体上的联会复合体长度和交叉位置进行量化也揭示了广泛交叉模式的保守性，包括强交叉干扰。我们建议稳定选择靶向重组或相关过程作为这些模式的解释。使用一种新的自动化方法对 1 号染色体上的联会复合体长度和交叉位置进行量化也揭示了广泛交叉模式的保守性，包括强交叉干扰。我们建议稳定选择靶向重组或相关过程作为这些模式的解释。使用一种新的自动化方法对 1 号染色体上的联会复合体长度和交叉位置进行量化也揭示了广泛交叉模式的保守性，包括强交叉干扰。我们建议稳定选择靶向重组或相关过程作为这些模式的解释。