Here, we report the first confirmed autochthonous tick-borne encephalitis case diagnosed in Moscow in 2016 and describe the detection of tick-borne encephalitis virus (TBEV) in ticks and small mammals in a Moscow park.

The paper includes data from two patients who were bitten by TBEV-infected ticks in Moscow city; one of these cases led to the development of the meningeal form of TBE. Both TBEV-infected ticks attacked patients in the same area. We collected ticks and trapped small mammals in this area in 2017. All samples were screened for the presence of pathogens causing tick-borne diseases by PCR. The TBEV-positive ticks and small mammals’ tissue samples were subjected to virus isolation. The sequencing of the complete polyprotein gene of the positive samples was performed.

A total of 227 questing ticks were collected. TBEV was detected in five specimens of Ixodes ricinus. We trapped 44 small mammals, mainly bank voles (Myodes glareolus) and pygmy field mice (Apodemus uralensis). Two samples of brain tissue from bank voles yielded a positive signal in RT-PCR for TBEV. We obtained six virus isolates from the ticks and brain tissue of a bank vole. Complete genome sequencing showed that the obtained isolates belong to the European subtype and have low diversity with sequence identities as high as 99.9%. GPS tracking showed that the maximum distance between the exact locations where the TBEV-positive ticks were collected was 185 m. We assume that the forest park had been free of TBEV and that the virus was recently introduced.

在这里，我们报告2016年在莫斯科诊断出的首例确诊的tick传播性脑炎脑炎病例，并描述了在莫斯科公园的tick和小型哺乳动物中检测到tick传播性脑炎病毒（TBEV）的情况。

该论文包括来自莫斯科市被TBEV感染的tick叮咬的两名患者的数据。其中一种情况导致了TBE的脑膜形式的发展。两种被TBEV感染的tick虫都袭击了同一地区的患者。我们在2017年收集了tick虫并困在该地区的小型哺乳动物中。通过PCR筛选了所有样本中是否存在引起tick虫传播疾病的病原体。对TBEV阳性s和小型哺乳动物的组织样本进行了病毒分离。对阳性样品的完整多蛋白基因进行测序。

总共收集了227个问号。在蓖麻的五个标本中检测到TBEV 。我们捕获了44种小型哺乳动物，主要是岸田鼠（Myodes glareolus）和侏儒田鼠（Apodemus uralensis）。来自银行田鼠的脑组织的两个样本在TBEV的RT-PCR中产生了阳性信号。我们从银行田鼠的the和脑组织中获得了六种病毒分离株。完整的基因组测序表明，所得分离株属于欧洲亚型，多样性低，序列同一性高达99.9％。GPS跟踪显示，收集TBEV阳性s的确切位置之间的最大距离为185 m。我们假定森林公园没有TBEV，并且该病毒是最近引入的。