Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.

中文翻译：

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用重组唾液酸转移酶直接荧光标记聚糖

糖基化是在许多蛋白质和脂质上发现的常见修饰。然而，直接检测这些完整生物分子上的聚糖一直是挑战。在这里，利用被称为直接荧光聚糖标记的荧光团缀合的唾液酸的酶促掺入，我们报道了糖蛋白上的N-和O-聚糖的标记和检测。该方法无需进行蛋白质印迹中费力的凝胶印迹和化学发光反应，即可检测特定的聚糖。该方法也可以与多种荧光染料一起使用。