Salmonella and Campylobacter are leading human foodborne pathogens commonly associated with poultry and poultry products, and several methods to control these pathogens have been applied to poultry production. This study was conducted to evaluate the effect of CALSPORIN, (CSP), a direct-fed microbial (DFM), and yeast cell wall (Saccharomyces cervisiae, IMW50, a mannanoligosaccharide (MOS)-based prebiotic, on performance, levels of Salmonella and Campylobacter in the feces, and intestinal histomorphometry in turkey poults. A 21-day battery cage study was conducted using 4 dietary treatments, including: an unsupplemented basal diet (corn and soybean-based) as negative control (NC); basal diet supplemented with 0.05% DFM; basal diet supplemented with 0.05% MOS; and basal diet supplemented with 0.05% mixture of DFM and MOS at equal proportions. Female Large White turkey poults (n = 336) were randomly distributed in 6 electrically-heated battery cages with 4 treatments and 12 replicates per treatment (7 poults per replicate pen). The first 16 pens were not inoculated with bacteria, while poults in pens 17 to 32 were orally challenged at day 7 with 105 CFU Salmonella Heidelberg and the poults in pens 33 to 48 were orally challenged at day 7 with 105 CFU Campylobacter jejuni. Feed consumption, body weight, and feed conversion ratio were measured weekly and at the end of the experiment. At day 21, fresh fecal samples from each pen were collected for Salmonella and Campylobacter enumeration and ileal tissue samples were collected from 1 bird per pen for histomorphology examination. DFM and MOS supplementation was accompanied with reduced levels of Salmonella shed by the treated birds compared to the control group, and with increased body weight (P ≤ 0.05). The surface area of villi increased in the MOS-supplemented group compared to the control group (P ≤ 0.05). There was a significant difference in V:C ratio between supplemented groups and control group (P ≤ 0.05). Based on these results, there is potential for CALSPORIN and IMW50 to reduce Salmonella shedding in feces, enhance ileal mucosal health, and improve growth performance of turkey poults.

中文翻译：

---

直接饲喂微生物和益生元对受到沙门氏菌和弯曲杆菌攻击的火鸡雏鸡的性能和肠道组织形态学的影响。

沙门氏菌和弯曲杆菌是常见的与家禽和家禽产品相关的主要人类食源性病原体，并且已经将几种控制这些病原体的方法应用于家禽生产。本研究旨在评估直接饲喂微生物 (DFM) CALSPORIN (CSP) 和酵母细胞壁 (Saccharomyces cervisiae, IMW50, 一种基于甘露寡糖 (MOS) 的益生元) 对性能、沙门氏菌水平和粪便中的弯曲杆菌和火鸡小鸡的肠道组织形态学。一项为期 21 天的电池笼研究使用 4 种饮食处理进行，包括：作为阴性对照 (NC) 的未添加基础饮食（玉米和大豆）；基础饮食中添加了0.05% DFM；基础日粮添加 0.05% MOS；基础日粮添加 0.05% DFM 和 MOS 等比例混合物。雌性大白火鸡雏鸡 (n = 336) 随机分布在 6 个电加热电池笼中，每个处理 4 个处理和 12 个重复（每个重复笔 7 个雏鸡）。前 16 个围栏没有接种细菌，而围栏 17 至 32 中的雏鸡在第 7 天用 105 CFU 海德堡沙门氏菌进行口服攻击，而围栏 33 至 48 中的雏鸡在第 7 天用 105 CFU 空肠弯曲杆菌进行口腔攻击。每周和实验结束时测量饲料消耗、体重和饲料转化率。在第 21 天，从每个围栏收集新鲜粪便样本用于沙门氏菌和弯曲杆菌计数，并从每个围栏的 1 只鸟中收集回肠组织样本用于组织形态学检查。与对照组相比，添加 DFM 和 MOS 的鸡只排出的沙门氏菌水平降低，体重增加（P ≤ 0.05）。与对照组相比，补充 MOS 组的绒毛表面积增加（P ≤ 0.05）。补充组与对照组的 V:C 比值有显着差异（P ≤ 0.05）。基于这些结果，CALSPORIN 和 IMW50 有可能减少粪便中沙门氏菌的脱落，增强回肠黏膜健康，并改善火鸡雏鸡的生长性能。