Probiotics are considered the preferred alternatives to antibiotics for growth promotion and disease prevention. Previous studies have confirmed that Diutina rugosa SD-17 has the potential as a probiotic. We evaluated the probiotic effect of D. rugosa SD-17 on 360 one-day-old Hy-line brown chickens that were divided into 2 groups, and each group contained 6 replicate pens with 30 birds per pen. The chickens were fed with basal diet supplemented with or without D. rugosa SD-17, and the effects of D. rugosa SD-17 on growth performance, intestine morphology, and immune status were assessed. Body weight was significantly improved from week 3 to 6 (P < 0.05), and the feed conversion ratio was significantly improved in weeks 1 and 2 (P < 0.001). The length of the duodenum was lengthened significantly in week 3 (P < 0.05), and supplementation of D. rugosa SD-17 significantly increased villus height and villus height to crypt depth ratio in the ileum in week 3 (P < 0.05). Expressions of tight-junction-related genes of zonula occludens-2 and occludin in the ileum were significantly increased (P < 0.05). The proliferation capacity of blood lymphocytes stimulated by concanavalin A was significantly enhanced (P < 0.05), and the proportion of helper T (Th) cells increased significantly (P < 0.05). Expressions of Th1 cell markers IL-2 and interferon (IFN)-γ and immune-related genes of IL-1β, transforming growth factor-β, and IFN-γ in ileum were significantly increased (P < 0.05). These results indicated that D. rugosa SD-17 improved the growth and regulated immunity of chickens, and could be optimized for use as a feed additive for livestock and poultry.

中文翻译：

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Diutina rugosa SD-17 对鸡生长性能、肠道形态和免疫状态的影响。

益生菌被认为是促进生长和预防疾病的抗生素的首选替代品。先前的研究已经证实 Diutina rugosa SD-17 具有作为益生菌的潜力。我们评估了 D. rugosa SD-17 对 360 只 1 日龄 Hy-line 棕色鸡的益生菌作用，将其分为 2 组，每组包含 6 个重复的圈，每圈 30 只鸡。给鸡饲喂添加或不添加 D. rugosa SD-17 的基础日粮，并评估 D. rugosa SD-17 对生长性能、肠道形态和免疫状态的影响。第3周至第6周体重显着改善（P < 0.05），第1周和第2周饲料转化率显着改善（P < 0.001）。第 3 周十二指肠长度显着延长（P < 0.05），并且补充 D. rugosa SD-17 显着增加了第 3 周回肠的绒毛高度和绒毛高度与隐窝深度的比值（P < 0.05）。zonula occludens-2和occludin紧密连接相关基因在回肠中的表达显着增加（P < 0.05）。刀豆球蛋白A刺激血淋巴细胞增殖能力显着增强（P < 0.05），辅助T（Th）细胞比例显着增加（P < 0.05）。回肠中Th1细胞标志物IL-2、干扰素（IFN）-γ和IL-1β、转化生长因子-β、IFN-γ免疫相关基因的表达显着升高（P < 0.05）。这些结果表明，D. rugosa SD-17改善了鸡的生长和调节免疫力，可以优化用作畜禽饲料添加剂。rugosa SD-17 在第 3 周显着增加了回肠绒毛高度和绒毛高度与隐窝深度的比值（P < 0.05）。zonula occludens-2和occludin紧密连接相关基因在回肠中的表达显着增加（P < 0.05）。刀豆球蛋白A刺激血淋巴细胞增殖能力显着增强（P < 0.05），辅助T（Th）细胞比例显着增加（P < 0.05）。回肠中Th1细胞标志物IL-2、干扰素（IFN）-γ和IL-1β、转化生长因子-β、IFN-γ免疫相关基因的表达显着升高（P < 0.05）。这些结果表明，D. rugosa SD-17改善了鸡的生长和调节免疫力，可以优化用作畜禽饲料添加剂。rugosa SD-17 在第 3 周显着增加了回肠绒毛高度和绒毛高度与隐窝深度的比值（P < 0.05）。zonula occludens-2和occludin紧密连接相关基因在回肠中的表达显着增加（P < 0.05）。刀豆球蛋白A刺激血淋巴细胞增殖能力显着增强（P < 0.05），辅助T（Th）细胞比例显着增加（P < 0.05）。回肠中Th1细胞标志物IL-2、干扰素（IFN）-γ和IL-1β、转化生长因子-β、IFN-γ免疫相关基因的表达显着升高（P < 0.05）。这些结果表明，D. rugosa SD-17改善了鸡的生长和调节免疫力，可以优化用作畜禽饲料添加剂。