Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, intellectual disability and so forth. Based on our previous studies, we here report a new functional signaling link between phospholipase D1 (PLD1) and protein kinase D1 (PKD1) in dendritic spine morphogenesis. Coimmunoprecipitation assays showed that PLD1 associates with PKD1. A series of knocking down and rescuing experiments demonstrated that PLD1 acts upstream of PKD1 in positively regulating dendritic spine morphogenesis. Using PLD1 inhibitor, we found that PLD1 activates PKD1 to promote dendritic spine morphogenesis. Thus, we further reveal the roles of the two different enzymes in neuronal development.

中文翻译：

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PLD1通过激活PKD1促进树突棘形态发生。

锥体神经元树突上的树突棘是兴奋性突触最重要的组成部分之一，其中兴奋性信息进行交换和整合。树突棘发育的缺陷与许多神经系统疾病密切相关，包括自闭症，智力障碍等。基于我们以前的研究，我们在这里报告了树突棘形态发生中磷脂酶D1（PLD1）和蛋白激酶D1（PKD1）之间的新功能性信号连接。免疫共沉淀测定表明PLD1与PKD1相关。一系列的敲除和挽救实验表明，PLD1在PKD1的上游积极调控树突状脊柱的形态发生。使用PLD1抑制剂，我们发现PLD1激活PKD1以促进树突棘形态发生。因此，