Previous work showed that peptides from duck eggs incubated for 15 D presented high total antioxidant activities. Here, this work explore the antioxidant activities of different segments, ZT1 (≤3 KD), ZT2 (≤10 KD), and ZT3 (≤30 KD), derived from duck embryo peptides and their protective effects against H2O2-induced oxidative damage in HepG2 cells. Peptides present no cytotoxicity to HepG2 cells. Moreover, ZT1 exhibits a higher ability to scavenge several radicals as well as stronger inhibition of H2O2-induced oxidative stress than ZT2 and ZT3. The activities of catalase and glutathione peroxidase as well as total superoxide dismutase increase in a concentration-dependent manner. Peptides are isolated from ZT1 and then subjected to LC-MS/MS to identify their sequences, followed by functional annotation, bioinformatics prediction, and hot-spot motif recognition. As a result, 413 potential functional peptides are identified, with some compounds exhibiting more than 1 function. This work will help for exploring bioactive substances in duck embryo eggs and enhance the utilization value of duck or other poultry eggs.

中文翻译：

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鸭胚肽对 H2O2 诱导的 HepG2 细胞氧化损伤的抗氧化活性和保护作用。

以前的工作表明，孵化 15 天的鸭蛋中的肽具有很高的总抗氧化活性。在这里，这项工作探讨了来自鸭胚肽的不同片段 ZT1 (≤3 KD)、ZT2 (≤10 KD) 和 ZT3 (≤30 KD) 的抗氧化活性及其对 H2O2 诱导的氧化损伤的保护作用。 HepG2 细胞。肽对 HepG2 细胞没有细胞毒性。此外，与 ZT2 和 ZT3 相比，ZT1 表现出更高的清除多种自由基的能力以及对 H2O2 诱导的氧化应激的更强抑制作用。过氧化氢酶和谷胱甘肽过氧化物酶以及总超氧化物歧化酶的活性以浓度依赖性方式增加。从 ZT1 中分离肽，然后进行 LC-MS/MS 鉴定其序列，然后进行功能注释、生物信息学预测、和热点主题识别。结果，确定了 413 种潜在的功能肽，其中一些化合物表现出超过 1 种功能。这项工作将有助于探索鸭胚蛋中的生物活性物质，提高鸭蛋或其他禽蛋的利用价值。