During cytokinesis, the organization of the spindle midzone and chromosome segregation is controlled by the central spindle, a microtubule cytoskeleton containing kinesin motors and non‐motor microtubule‐associated proteins. The anaphase spindle elongation 1/protein regulator of cytokinesis 1/microtubule associated protein 65 (Ase1/PRC1/MAP65) family of microtubule‐bundling proteins are key regulators of central spindle assembly, mediating microtubule crosslinking and spindle elongation in the midzone. Ase1/PRC1/MAP65 serves as a complex regulatory platform for the recruitment of other midzone proteins at the spindle midzone. Herein, we summarize recent advances in understanding of the structural domains and molecular kinetics of the Ase1/PRC1/MAP65 family. We summarize the regulatory network involved in post‐translational modifications of Ase1/PRC1 by cyclin‐dependent kinase 1 (Cdk1), cell division cycle 14 (Cdc14) and Polo‐like kinase 1 (Plk1) and also highlight multiple functions of Ase1/PRC1 in central spindle organization, spindle elongation and cytokinesis during cell division.

中文翻译：

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Ase1/PRC1/MAP65 家族在中心主轴组装中的机制

在胞质分裂期间，纺锤体中区的组织和染色体分离由中心纺锤体控制，中心纺锤体是一种含有驱动蛋白马达和非运动微管相关蛋白的微管细胞骨架。后期纺锤体伸长 1/胞质分裂蛋白调节剂 1/微管相关蛋白 65 (Ase1/PRC1/MAP65) 微管捆绑蛋白家族是中心纺锤体组装的关键调节因子，介导微管交联和纺锤体在中区的伸长。Ase1/PRC1/MAP65 作为一个复杂的调控平台，用于在纺锤体中区招募其他中区蛋白。在此，我们总结了对 Ase1/PRC1/MAP65 家族的结构域和分子动力学的理解的最新进展。