AIMS The exact pathogenesis of neuromyelitis optica spectrum disorder (NMOSD) remains unclear. A variety of cytokines are involved, but few studies have been performed to explore the novel roles of interleukin-22 (IL-22) and interleukin-35 (IL-35) in NMOSD. Therefore, this study was designed to investigate serum levels of IL-22 and IL-35, and their correlations with clinical and laboratory characteristics in NMOSD. METHODS We performed a cross-section study, 18 patients with acute NMOSD, 23 patients with remission NMOSD, and 36 healthy controls were consecutively enrolled. Serum levels of IL-22 and IL-35 were measured by enzyme-linked immunosorbent assay (ELISA). The correlations between serum IL-22 and IL-35 levels and clinical and laboratory characteristics were evaluated by Spearman's rank or Pearson's correlation coefficient. RESULTS The serum levels of IL-22 and IL-35 were significantly lower in patients with acute NMOSD and remission NMOSD than in healthy controls (IL-22: 76.96 ± 13.62 pg/mL, 87.30 ± 12.79 pg/mL, and 94.02 ± 8.52 pg/mL, respectively, P < .0001; IL-35: 45.52 ± 7.04 pg/mL, 57.07 ± 7.68 pg/mL, and 60.05 ± 20.181 pg/mL, respectively, P < .0001). Serum levels of IL-35 were negatively correlated with EDSS scores and cerebrospinal fluid protein levels (r = -.5438, P = .0002 and r = -.3523, P = .0258, respectively) in all patients. CONCLUSIONS Lower serum levels of IL-22 and IL-35 are associated with disease status in NMOSD. Additionally, lower serum levels of IL-35 are associated with disease severity in NMOSD.

中文翻译：

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较低的血清白细胞介素 22 和白细胞介素 35 水平与视神经脊髓炎谱系疾病的疾病状态相关。


目的 视神经脊髓炎谱系障碍 (NMOSD) 的确切发病机制仍不清楚。涉及多种细胞因子，但很少有研究探索白细胞介素 22 (IL-22) 和白细胞介素 35 (IL-35) 在 NMOSD 中的新作用。因此，本研究旨在探讨 NMOSD 血清 IL-22 和 IL-35 水平及其与临床和实验室特征的相关性。方法采用横断面研究，连续纳入18例急性NMOSD患者、23例缓解期NMOSD患者和36例健康对照。通过酶联免疫吸附测定（ELISA）测量IL-22和IL-35的血清水平。通过Spearman等级或Pearson相关系数评估血清IL-22和IL-35水平与临床和实验室特征之间的相关性。结果 急性NMOSD和缓解期NMOSD患者血清IL-22和IL-35水平显着低于健康对照（IL-22：76.96±13.62 pg/mL、87.30±12.79 pg/mL和94.02±8.52） pg/mL，分别为 P < .0001；IL-35：分别为 45.52 ± 7.04 pg/mL、57.07 ± 7.68 pg/mL 和 60.05 ± 20.181 pg/mL，P < .0001）。所有患者的血清 IL-35 水平与 EDSS 评分和脑脊液蛋白水平呈负相关（分别为 r = -.5438，P = .0002 和 r = -.3523，P = .0258）。结论 较低的血清 IL-22 和 IL-35 水平与 NMOSD 的疾病状态相关。此外，较低的血清 IL-35 水平与 NMOSD 的疾病严重程度相关。