N⁶-methyladenosine (m⁶A) emerges as an important modification in eukaryotic mRNAs. m⁶A has first been reported in 1974, and its functional significance in mammalian gene regulation and importance for proper development have been well established. An arsenal of writer, eraser, and reader proteins accomplish deposition, removal, and interpretation of the m⁶A mark, resulting in dynamic function. This led to the concept of an epitranscriptome, the compendium of RNA species with chemical modification of the nucleobases in the cell, in analogy to the epigenome. While m⁶A has long been known to also exist in plant mRNAs, proteins involved in m⁶A metabolism have only recently been detected by mutant analysis, homology search, and mRNA interactome capture in the reference plant Arabidopsis thaliana. Dysregulation of the m⁶A modification causes severe developmental abnormalities of leaves and roots and altered timing of reproductive development. Furthermore, m⁶A modification affects viral infection. Here, we discuss recent progress in identifying m⁶A sites transcriptome-wide, in identifying the molecular players involved in writing, removing, and reading the mark, and in assigning functions to this RNA modification in A. thaliana. We highlight similarities and differences to m⁶A modification in mammals and provide an outlook on important questions that remain to be addressed.

中文翻译：

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标记RNA：拟南芥中m6A的作者，读者和功能。

N ^6-甲基腺苷（m ⁶ A）作为真核mRNA中的重要修饰而出现。m ⁶ A最早在1974年被报道，其在哺乳动物基因调控中的功能意义和对适当发育的重要性已得到充分确立。大量的书写，擦除和读取蛋白可以完成m ⁶ A标记的沉积，去除和解释，从而产生动态功能。这导致了表观转录组的概念，即类似于表观基因组的RNA物种纲要，对细胞中的核碱基进行了化学修饰。虽然早就知道m ⁶ A也存在于植物mRNA中，但参与m ^6的蛋白质仅在参考植物拟南芥中通过突变分析，同源性搜索和mRNA相互作用组捕获才检测到新陈代谢。m ⁶ A修饰的失调导致叶和根的严重发育异常和生殖发育时间的改变。此外，m ⁶ A修饰会影响病毒感染。在这里，我们讨论了在整个转录组中鉴定m ⁶ A位点，鉴定参与书写，去除和阅读标记的分子参与者以及在拟南芥中对该RNA修饰赋予功能的最新进展。我们强调与m ^6的异同哺乳动物的改良，并为尚待解决的重要问题提供了前景。