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Respond of clay amendment in chicken manure composts to understand the antibiotic resistant bacterial diversity and its correlation with physicochemical parameters
Journal of Cleaner Production ( IF 9.7 ) Pub Date : 2019-07-22 , DOI: 10.1016/j.jclepro.2019.117715
Mukesh Kumar Awasthi , Hongyu Chen , Tao Liu , Sanjeev Kumar Awasthi , Quan Wang , Xiuna Ren , Yumin Duan , Zengqiang Zhang

Clay has a positive influence on organic matter degradation and nitrogen conservation during chicken manure composting, but its effects on the diversity of antibiotic-resistant bacterial are unclear. The impact of the clay amendment (0% (T1), 2% (T2), 4% (T3), 6% (T4), 8% (T5) and 10% (T6) on the environmental risk of antibiotic-resistant bacteria abundance during chicken manure composting was investigated. The results showed that 4% clay increased the relative abundances of antibiotic-resistant bacterial, while 8% and 10% clay additions significantly reduced the total relative abundances of antibiotic-resistant bacterial in the compost, where the relative abundances of antibiotic-resistant bacterial was 0.46 log lower than the control. Clay mainly affects the distribution of antibiotic-resistant bacterial by influencing the relative abundances of Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes phylum. The maximum dosage of the clay amendment had a more persistent impact on the antibiotic-resistant bacterial community. However, bacterial abundance could be classified according to the composting time under diverse treatments. In the present study, the abundance of the antibiotic-resistant bacterial has a considerable impact on the alteration of the physicochemical parameters during composting. The correlations between the physicochemical parameters (temperature, carbon/nitrogen ratio, and gaseous emissions) and antibiotic-resistant bacterial were more significant than pH and germination index.



中文翻译:

鸡粪堆肥中粘土改良剂的响应以了解抗药性细菌多样性及其与理化参数的关系

粘土对鸡粪堆肥过程中的有机物降解和氮保持有积极影响,但对抗生素抗性细菌多样性的影响尚不清楚。粘土改良剂(0%(T1),2%(T2),4%(T3),6%(T4),8%(T5)和10%(T6)对耐抗生素环境风险的影响研究了鸡粪堆肥过程中细菌的丰度,结果表明:4%的粘土增加了抗药性细菌的相对丰度,而8%和10%的粘土添加量则显着降低了堆肥中抗生素抗性细菌的总相对丰度。抗生素抗性细菌的相对丰度比对照组低0.46 log,粘土主要通过影响细菌的相对丰度来影响抗生素抗性细菌的分布。FirmicutesProteobacteriaActinobacteriaBacteroidetes phylum。粘土改良剂的最大剂量对耐抗生素细菌群落具有更持久的影响。但是,可以根据不同处理条件下的堆肥时间对细菌丰度进行分类。在本研究中,抗生素抗性细菌的丰度对堆肥过程中理化参数的改变有相当大的影响。理化参数(温度,碳/氮比和气体排放)与抗药性细菌之间的相关性比pH和发芽指数更重要。

更新日期:2019-07-22
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