The treatment of colored effluents has been addressed using different methodologies, such as the coupling of adsorption to solid-state fermentation (SSF). This work shows for the first time the use of an agricultural by-product (corncob) as the adsorbent for the removal of the binary mixture of dyes, brilliant blue and allura red, and their subsequent decolorization by white-rot fungi. , and their consortium were the selected fungal species for carrying out the second step of this bioprocess. A 2 full factorial design was adopted, obtaining a maximum decolorization of 90.8 ± 4.1% with at a 20:1 carbon:nitrogen ratio, 80% moisture and the absence of inducer. Moreover, the effect of different carbon and nitrogen sources was evaluated; yeast extract was discovered to be the most efficient nitrogen source, while the supplement of a carbon source was unnecessary to reach high levels of decolorization. Regarding the influence of the inducer, it was found that although copper addition favored enzyme levels of laccase (Lac), no positive effect was observed in either the decolorization rate of the dye mixture or the manganese peroxidase (MnP) activity, and a maximum of 19.8 ± 2.1 U Lac gds and 5.4 ± 1.3 U MnP gds was obtained. These results suggest that the coupled adsorption-SSF methodology using corncob and white-rot fungi represents a promising strategy for the treatment of effluents polluted with dye mixtures.

中文翻译：

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铜和不同碳氮源对工业染料混合物固态发酵脱色的影响


已使用不同的方法来处理有色废水，例如吸附与固态发酵（SSF）的耦合。这项工作首次展示了使用农业副产品（玉米芯）作为吸附剂去除染料（亮蓝和诱惑红）的二元混合物，以及随后通过白腐真菌进行脱色。和他们的联合体是被选择的用于进行该生物过程第二步的真菌物种。采用2全因子设计，在碳氮比20:1、水分80%且不存在诱导剂的情况下，获得了90.8±4.1%的最大脱色率。此外，还评估了不同碳源和氮源的影响；人们发现酵母提取物是最有效的氮源，而无需补充碳源即可达到高水平的脱色。关于诱导剂的影响，发现虽然铜的添加有利于漆酶（Lac）的酶水平，但在染料混合物的脱色率或锰过氧化物酶（MnP）活性方面没有观察到积极影响，并且最大获得 19.8 ± 2.1 U Lac gds 和 5.4 ± 1.3 U MnP gds。这些结果表明，使用玉米芯和白腐真菌的耦合吸附-SSF 方法代表了处理染料混合物污染的废水的一种有前景的策略。