The effect of exogenous electrical stimulation on cell viability, attachment, growth, and neurogenesis was examined using PC12 cells in microfibrous viscose-rayon scaffolds immersed in culture medium. The scaffolds were applied either in their nonconductive state or after coating the fibres with 200 nm of gold to give a scaffold sheet resistivity of (13 ± 1.3) Ω square⁻¹. The cells were treated for 12 days using direct current electrical stimulation of 2 h per day. No cytotoxic effects were observed when up to 500 mV (8.3 mV mm⁻¹) was applied to the scaffolds without gold, or when up to 100 mV (1.7 mV mm⁻¹) was applied to the scaffolds with gold. Compared with unstimulated cells, whereas electrical stimulation significantly enhanced cell growth and attachment in the nonconductive scaffolds without gold, similar effects were not found for the conductive scaffolds with gold. Neural differentiation in the presence of nerve growth factor was improved by electrical stimulation in both scaffolds; however, neurite development and the expression of key differentiation markers were greater in the nonconductive scaffolds without gold than in the scaffolds with gold. Application of the same current to scaffolds with and without gold led to much higher levels of neurogenesis in the scaffolds without gold. This work demonstrates that substantial benefits in terms of cell growth and neural differentiation can be obtained using electric fields exerted across nonconductive microfibrous scaffolds, and that this approach to electrical stimulation can be more effective than when the stimulus is applied to cells on conductive scaffolds.

中文翻译：

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使用导电和非导电微纤维支架电刺激细胞生长和神经发生

使用浸在培养基中的微纤维粘胶人造丝支架中的PC12细胞检查了外源电刺激对细胞活力，附着，生长和神经发生的影响。支架以其非导电状态或在纤维上涂覆200 nm的金后涂覆，以提供（13±1.3）Ωsquare ^-1的支架薄层电阻率。使用每天2小时的直流电刺激将细胞处理12天。当对不含金的支架施加高达500 mV（8.3 mV mm ^-1）或高达100 mV（1.7 mV mm ^-1）时，未观察到细胞毒性作用）用金涂到脚手架上。与未刺激的细胞相比，电刺激显着增强了不含金的非导电支架中的细胞生长和附着，但对于含金的导电支架未发现相似的作用。两种支架均通过电刺激改善了神经生长因子存在下的神经分化。然而，在没有金的非导电支架中，与在有金的支架中相比，神经突的发育和关键分化标志物的表达更大。在有和没有金的支架上施加相同电流会导致在没有金的支架中更高水平的神经发生。