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Reduced sialyl-Lewisx on salivary MUC7 from patients with burning mouth syndrome.
Molecular Omics ( IF 3.0 ) Pub Date : 2019-10-07 , DOI: 10.1039/c9mo00061e Shikha Acharya 1 , Chunsheng Jin , Johan Bylund , Qiujin Shen , Masood Kamali-Moghaddam , Mats Jontell , Anette Carlén , Niclas G Karlsson
Molecular Omics ( IF 3.0 ) Pub Date : 2019-10-07 , DOI: 10.1039/c9mo00061e Shikha Acharya 1 , Chunsheng Jin , Johan Bylund , Qiujin Shen , Masood Kamali-Moghaddam , Mats Jontell , Anette Carlén , Niclas G Karlsson
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We analysed and compared MUC7 O-glycosylation and inflammatory biomarkers in saliva from female patients with burning mouth syndrome (BMS) and gender/age-matched controls. Oligosaccharides from salivary MUC7 from BMS and controls were released. Inflammatory mediators were measured by multiplex proximity extension assay. Presence of sialyl-Lewisx (Si-Lex) epitope on MUC7 was confirmed using Western blot. MUC7 O-glycans and measured inflammatory biomarkers were found to be similar between BMS and controls. However, oligosaccharides sialyl-Lewisx (Si-Lex) was found to be reduced in samples from BMS patients. Positive correlation (combined patients and controls) was found between levels of C-C motif chemokine 19 (CCL-19) and the amount of core-2 oligosaccharides on MUC7 as well as fractalkine (CX3CL1) and level of sialylation. Patients with BMS were shown to represent a heterogeneous group in terms of inflammatory biomarkers. This indicates that BMS patients could be further stratified on the basis of low-level inflammation. The results furthermore indicate that reduced sialylation of MUC7, particularly Si-Lex, may be an important feature in patients with BMS. However, the functional aspects and potential involvement in immune regulation of Si-Lex remains unclear. Our data suggests a chemokine driven alteration of MUC7 glycosylation.
中文翻译：
口腔灼热症患者唾液中MUC7的唾液酸化-Lewisx减少。
我们分析和比较了患有烧嘴综合征（BMS）和性别/年龄相匹配的对照的女性患者唾液中的MUC7 O-糖基化和炎症生物标志物。释放了来自BMS唾液MUC7和对照的寡糖。通过多重接近扩展测定法测量炎性介质。使用蛋白质印迹法证实了MUC7上存在唾液酸化-Lewisx（Si-Lex）表位。发现BMS和对照之间的MUC7 O-聚糖和测得的炎症生物标志物相似。但是，发现BMS患者的样品中的低聚糖唾液酸化-Lewisx（Si-Lex）减少了。发现CC基序趋化因子19（CCL-19）的水平与MUC7以及fractalkine（CX3CL1）上的core-2寡糖的量和唾液酸化水平之间呈正相关（患者与对照合并）。BMS患者在炎症生物标志物方面表现出异质性。这表明BMS患者可以根据低度炎症进一步分层。结果进一步表明，MUC7，特别是Si-Lex的唾液酸化减少可能是BMS患者的重要特征。但是，Si-Lex的免疫调节的功能方面和潜在的参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。Si-Lex的免疫调节的功能方面和潜在参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。Si-Lex的免疫调节的功能方面和潜在的参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。
更新日期：2019-10-07
中文翻译：
口腔灼热症患者唾液中MUC7的唾液酸化-Lewisx减少。
我们分析和比较了患有烧嘴综合征（BMS）和性别/年龄相匹配的对照的女性患者唾液中的MUC7 O-糖基化和炎症生物标志物。释放了来自BMS唾液MUC7和对照的寡糖。通过多重接近扩展测定法测量炎性介质。使用蛋白质印迹法证实了MUC7上存在唾液酸化-Lewisx（Si-Lex）表位。发现BMS和对照之间的MUC7 O-聚糖和测得的炎症生物标志物相似。但是，发现BMS患者的样品中的低聚糖唾液酸化-Lewisx（Si-Lex）减少了。发现CC基序趋化因子19（CCL-19）的水平与MUC7以及fractalkine（CX3CL1）上的core-2寡糖的量和唾液酸化水平之间呈正相关（患者与对照合并）。BMS患者在炎症生物标志物方面表现出异质性。这表明BMS患者可以根据低度炎症进一步分层。结果进一步表明，MUC7，特别是Si-Lex的唾液酸化减少可能是BMS患者的重要特征。但是，Si-Lex的免疫调节的功能方面和潜在的参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。Si-Lex的免疫调节的功能方面和潜在参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。Si-Lex的免疫调节的功能方面和潜在的参与尚不清楚。我们的数据表明趋化因子驱动的MUC7糖基化改变。
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