Genistein (GEN), a type of soy isoflavones, is similar to estrogen structurally and functionally. The effects of dietary gen on the reproductive performance and bone status of breeder hens were investigated. A total pf 720 laying broiler breeder (LBB) hens were randomly allocated into 3 groups with supplemental dietary GEN doses (0, 40, 400 mg/kg). Each treatment has 8 replicates of 30 birds. The results indicated that supplemental GEN significantly improved the egg production and eggshell strength of LBB hens. Dietary GEN was deposited into the egg yolk, which decreased malonaldehyde in the follicle and egg yolk. The levels of vitellogenin (VTG), progesterone, and follicle-stimulating hormone in the serum of GEN-treated groups were elevated compared with the control group. Furthermore, GEN treatment downregulated the mRNA expression of insulin-like growth factor binding protein in the fallopian tube, whereas 40 mg/kg GEN treatment upregulated estrogen receptor α expression. Both the mRNA expression of VTG-II in the liver and mRNA expression of amphiregulin in the fallopian tube were upregulated after 40 and 400 mg/kg GEN treatment. In the 400 mg/kg GEN-treated group, the levels of calcitonin and alkaline phosphatase in the serum were increased compared with the control group, which was consistent with the increased levels of calcium and phosphorus in the tibia. Supplemental GEN (400 mg/kg) improved the tibia strength of LBB hens, whereas 40 mg/kg GEN had better effects on laying performance. In summary, dietary GEN could improve the egg production and quality, as well as the bone status of LBB hens during the late egg-laying period.

中文翻译：

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金雀异黄素可改善种鸡在产蛋后期的繁殖性能和骨骼状况。

金雀异黄素 (GEN) 是一种大豆异黄酮，在结构和功能上与雌激素相似。研究了日粮基因对种鸡繁殖性能和骨骼状态的影响。总共 pf 720 只蛋鸡种鸡 (LBB) 母鸡被随机分配到 3 组中，补充日粮 GEN 剂量 (0, 40, 400 mg/kg)。每个处理有 30 只鸡 8 个重复。结果表明，补充 GEN 显着提高了 LBB 母鸡的产蛋量和蛋壳强度。日粮 GEN 沉积在蛋黄中，从而减少了卵泡和蛋黄中的丙二醛。与对照组相比，GEN治疗组血清中的卵黄素（VTG）、孕酮和促卵泡激素水平升高。此外，GEN处理下调输卵管中胰岛素样生长因子结合蛋白的mRNA表达，而40 mg/kg GEN处理上调雌激素受体α表达。在 40 和 400 mg/kg GEN 处理后，肝脏中 VTG-II 的 mRNA 表达和输卵管中双调蛋白的 mRNA 表达均上调。与对照组相比，400 mg/kg GEN处理组血清中降钙素和碱性磷酸酶水平升高，这与胫骨钙、磷水平升高一致。补充 GEN (400 mg/kg) 提高了 LBB 母鸡的胫骨强度，而 40 mg/kg GEN 对产蛋性能有更好的影响。总之，日粮GEN可以提高产蛋量和质量，